This study aims at the assessment of total zinc contents, water zinc extract contents and zinc-protein profile in medicinal plants traditionally used for diabetes treatment. While zinc-protein profile was screened in plant samples using the online coupling of size exclusion chromatography-inductively coupled plasma-mass spectrometry (SEC-ICP-MS), total zinc contents and zinc water extract contents were determined using inductively coupled plasma-optical emission spectrometry (ICP-OES). The analysis of two certified reference materials with similar matrix for total zinc content revealed recovery values of 97.1% and 100.5% while the average of recovery of the summed Zn concentrations from protein fractions compared to total Zn was 103.0 ± 4.8%. Based on the FAO/WHO classification, Sesamum indicum, Nigella sativa, Trigonella Foenum-graecum and Pennisetum glaucum are classified as highest Zn-content. For protein profile, zinc was quantified in 330-430 and 50-60 kDa fractions of all examined plants while no contents were quantified in the inorganic fraction 0.05-0.4 kDa of all plant species. Also, only three plant species recorded Zn contents in the phytate fraction (0.9-1.5 kDa fraction). The fruits of the Momordica Charantia and the Citrullus colocynthis were with the highest extractable zinc concentration; 13.55 ± 0.45 and 10.08 ± 0.63 mg/kg, respectively. The highest Zinc capturing capacity was reported for the 50-60 and 70-87 KDa protein fractions.

中文翻译：

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传统上用于治疗糖尿病的药用植物中总锌含量和锌蛋白谱的研究。

这项研究旨在评估传统上用于治疗糖尿病的药用植物中的总锌含量，水锌提取物含量和锌-蛋白质谱。使用尺寸排阻色谱-电感耦合等离子体质谱（SEC-ICP-MS）在线耦合筛选植物样品中的锌蛋白特征时，使用电感耦合等离子体发射光谱法测定了总锌含量和锌水提取物含量光谱仪（ICP-OES）。两种具有相似基质的认证参考物质的总锌含量分析显示回收率分别为97.1％和100.5％，而蛋白质组分中总锌浓度的回收率与总锌的平均值为103.0±4.8％。根据粮农组织/世卫组织的分类标准，芝麻，Nigella sativa，三角叶草和青草被认为是最高的锌含量。对于蛋白质谱，在所有被检植物的330-430和50-60 kDa馏分中定量了锌，而在所有植物物种的0.05-0.4 kDa的无机馏分中没有定量含量。同样，只有三种植物在植酸盐级分（0.9-1.5 kDa级分）中记录了锌含量。苦瓜和瓜（Citrullus colocynthis）的果实具有最高的可提取锌浓度。分别为13.55±0.45和10.08±0.63 mg / kg。据报道，对于50-60和70-87 KDa的蛋白质，锌的捕获能力最高。锌在所有被检植物的330-430和50-60 kDa馏分中定量，而在所有植物物种的0.05-0.4 kDa的无机馏分中含量均未定量。同样，只有三种植物在植酸盐级分（0.9-1.5 kDa级分）中记录了锌含量。苦瓜和瓜（Citrullus colocynthis）的果实具有最高的可提取锌浓度。分别为13.55±0.45和10.08±0.63 mg / kg。据报道，对于50-60和70-87 KDa的蛋白质，锌的捕获能力最高。锌在所有被检植物的330-430和50-60 kDa馏分中定量，而在所有植物物种的0.05-0.4 kDa的无机馏分中含量均未定量。同样，只有三种植物在植酸盐级分（0.9-1.5 kDa级分）中记录了锌含量。苦瓜和瓜（Citrullus colocynthis）的果实具有最高的可提取锌浓度。分别为13.55±0.45和10.08±0.63 mg / kg。据报道，对于50-60和70-87 KDa的蛋白质，锌的捕获能力最高。