Multiparental genetic mapping populations such as nested-association mapping (NAM) have great potential for investigating quantitative traits and associated genomic regions leading to rapid discovery of candidate genes and markers. To demonstrate the utility and power of this approach, two NAM populations, NAM_Tifrunner and NAM_Florida-07, were used for dissecting genetic control of 100-pod weight (PW) and 100-seed weight (SW) in peanut. Two high-density SNP-based genetic maps were constructed with 3341 loci and 2668 loci for NAM_Tifrunner and NAM_Florida-07, respectively. The quantitative trait locus (QTL) analysis identified 12 and 8 major effect QTLs for PW and SW, respectively, in NAM_Tifrunner, and 13 and 11 major effect QTLs for PW and SW, respectively, in NAM_Florida-07. Most of the QTLs associated with PW and SW were mapped on the chromosomes A05, A06, B05 and B06. A genomewide association study (GWAS) analysis identified 19 and 28 highly significant SNP-trait associations (STAs) in NAM_Tifrunner and 11 and 17 STAs in NAM_Florida-07 for PW and SW, respectively. These significant STAs were co-localized, suggesting that PW and SW are co-regulated by several candidate genes identified on chromosomes A05, A06, B05, and B06. This study demonstrates the utility of NAM population for genetic dissection of complex traits and performing high-resolution trait mapping in peanut.

中文翻译：

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基于嵌套关联映射（NAM）的遗传解剖揭示了花生（花生）种子和荚果重量的候选基因。

诸如巢式关联作图（NAM）等多亲遗传作图种群在调查数量性状和相关的基因组区域方面具有巨大的潜力，从而可以迅速发现候选基因和标记。为了证明这种方法的实用性和效力，使用了两个NAM种群NAM_Tifrunner和NAM_Florida-07来剖析花生中100荚重（PW）和100种子重（SW）的遗传控制。分别为NAM_Tifrunner和NAM_Florida-07构建了两个基于SNP的高密度遗传图谱，分别具有3341个基因座和2668个基因座。定量性状基因座（QTL）分析在NAM_Tifrunner中分别确定了PW和SW的12个和8个主要效应QTL，在NAM_Florida-07中分别确定了PW和SW的13个和11个主要效应QTL。与PW和SW相关的大多数QTL位于染色体A05，A06，B05和B06染色体上。全基因组关联研究（GWAS）分析确定了PW和SW分别在NAM_Tifrunner中的19个和28个高度显着的SNP性状关联（STA）和NAM_Florida-07中的11个和17个STA。这些重要的STA共定位，表明PW和SW受染色体A05，A06，B05和B06上鉴定的几种候选基因共同调控。这项研究证明了NAM群体对花生复杂性状的遗传解剖和进行高分辨率性状作图的实用性。这些重要的STA共定位，表明PW和SW受染色体A05，A06，B05和B06上鉴定的几种候选基因共同调控。这项研究证明了NAM群体对花生复杂性状的遗传解剖和进行高分辨率性状作图的实用性。这些重要的STA共定位，表明PW和SW受染色体A05，A06，B05和B06上鉴定的几种候选基因共同调控。这项研究证明了NAM群体对花生复杂性状的遗传解剖和进行高分辨率性状作图的实用性。