Increase in the current variance in bilayer lipid membranes near phase transition as a result of the occurrence of hydrophobic defects.,Biochimica et Biophysica Acta (BBA) - Biomembranes

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Increase in the current variance in bilayer lipid membranes near phase transition as a result of the occurrence of hydrophobic defects.
Biochimica et Biophysica Acta (BBA) - Biomembranes ( IF 2.8 ) Pub Date : 2019-12-06 , DOI: 10.1016/j.bbamem.2019.183147
A A Anosov ₁ , E Yu Smirnova ₂ , A A Sharakshane ₃ , E A Nikolayeva ₂ , Yu S Zhdankina ₂

Affiliation

Most researchers associate the increase in the permeability of lipid bilayers of artificial and biological membranes observed in various experiments with the formation of hypothetical hydrophobic and hydrophilic pores. Although the existence of hydrophobic defects, as the first stage of the formation of a hydrophilic pore, was hypothesized decades ago from electroporation experiments, the difficulty of describing this stage is determined by the lack of experimental data confirming the existence or at least associated with hydrophobic pores. We explored the increase in the current variance through the lipid membrane, observed when approaching the phase transition from the side of high temperatures, and have associated it with capacitive currents arising in response to the formation of hydrophobic pores. Assuming that the number of hydrophobic pores in a membrane follows a Poisson distribution, and thus, the mean number of hydrophobic pores is equal to the variance of that number, we used the measurements of the membrane current variance to evaluate the number of hydrophobic pores. Analysis of experimental data within this model allows us to estimate the number of hydrophobic pores at or above the phase transition and shows that the number of hydrophobic pores in a membrane close to the phase transition increased 20 times compared to the number of hydrophobic pores existing in the membrane far from the melting transition.

中文翻译：

由于发生疏水性缺陷，双层脂质膜中相变附近电流变化的增加。

大多数研究人员将在各种实验中观察到的人工膜和生物膜的脂双层的渗透性增加与假想的疏水孔和亲水孔的形成联系起来。尽管疏水缺陷的存在是亲水性孔形成的第一步，但几十年前已通过电穿孔实验进行了假设，但由于缺乏确定疏水性或至少与疏水性相关的实验数据，难以描述该缺陷。毛孔。我们探索了通过脂质膜的电流变化的增加，当从高温侧接近相变时观察到了这种变化，并将其与响应于疏水孔形成而产生的电容性电流相关联。假设膜中疏水孔的数量遵循泊松分布，因此，疏水孔的平均数量等于该数量的方差，我们使用膜电流方差的测量值来评估疏水孔的数量。在该模型中对实验数据进行分析，使我们能够估算出相变或相变以上的疏水孔的数量，并表明与相变中存在的疏水孔的数量相比，接近相变的膜中疏水孔的数量增加了20倍。膜远离熔融转变。我们使用膜电流变化的测量值来评估疏水孔的数量。在该模型中对实验数据进行分析，使我们能够估算出相变或相变以上的疏水孔的数量，并表明与相变中存在的疏水孔的数量相比，接近相变的膜中疏水孔的数量增加了20倍。膜远离熔融转变。我们使用膜电流变化的测量值来评估疏水孔的数量。在该模型中对实验数据进行分析，使我们能够估算出相变或相变以上的疏水孔的数量，并表明与相变中存在的疏水孔的数量相比，接近相变的膜中疏水孔的数量增加了20倍。膜远离熔融转变。

更新日期：2019-12-06

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