Abstract We introduce a novel magnetically coupled mixer drive for biotechnological, pharmaceutical and chemical applications requiring high-grade aseptic conditions. The proposed design enables its application in larger volume bioreactors compared to traditional magnetic drives, due to a possibility of introducing higher power quantities per individual rotor, while ensuring higher mixing intensity. In the following study, we provide information on the design aspects of the novel magnetic drive and demonstrate the principles behind both theoretical and experimental maximal torque and other key working parameter evaluation. Based on the mentioned principles, magnetic mixer drives were developed for laboratory (5 dm3) and pilot-scale (15 m3) bioreactors. The specific power inputs relative to the agitation rates for the mentioned mixers were experimentally determined in water for two impeller types: six-bladed Rushton and three-bladed Pitched-blade turbines. The performance of the novel magnetic drive was evaluated during Kluyveromyces marxianus cultivations utilizing whey (a well known byproduct of the dairy industry) as the main substrate source in the 15 m3 bioreactor. The obtained results show that the novel magnetic drive can be successfully applied in 15 m3 bioreactors, while providing sufficient mixing of the cultivation broth for biomass growth.

中文翻译：

---

新型磁耦合混合器驱动器在高达 15 m3 的生物反应器中的应用

摘要 我们介绍了一种用于需要高级无菌条件的生物技术、制药和化学应用的新型磁耦合混合器驱动器。与传统磁驱动器相比，所提出的设计使其能够应用于更大容量的生物反应器，因为每个转子有可能引入更高的功率，同时确保更高的混合强度。在接下来的研究中，我们提供了有关新型磁驱动器设计方面的信息，并展示了理论和实验最大扭矩以及其他关键工作参数评估背后的原理。基于上述原理，为实验室 (5 dm3) 和中试规模 (15 m3) 生物反应器开发了磁性混合器驱动器。与上述混合器的搅拌速率相关的特定功率输入是在水中实验确定的两种叶轮类型：六叶 Rushton 和三叶斜叶涡轮。在 15 立方米生物反应器中利用乳清（一种众所周知的乳制品工业副产品）作为主要底物来源，在马克斯克鲁维酵母培养期间评估了新型磁驱动器的性能。获得的结果表明，新型磁力驱动器可以成功应用于 15 m3 生物反应器，同时为生物质生长提供足够的培养液混合。在 15 立方米生物反应器中利用乳清（一种众所周知的乳制品工业副产品）作为主要底物来源，在马克斯克鲁维酵母培养期间评估了新型磁驱动器的性能。获得的结果表明，新型磁力驱动器可以成功应用于 15 m3 生物反应器，同时为生物质生长提供足够的培养液混合。在 15 立方米生物反应器中利用乳清（一种众所周知的乳制品工业副产品）作为主要底物来源，在马克斯克鲁维酵母培养期间评估了新型磁驱动器的性能。获得的结果表明，新型磁力驱动器可以成功应用于 15 m3 生物反应器，同时为生物质生长提供足够的培养液混合。