Benchtop NMR for Online Reaction Monitoring of the Biocatalytic Synthesis of Aromatic Amino Alcohols,ChemCatChem

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Benchtop NMR for Online Reaction Monitoring of the Biocatalytic Synthesis of Aromatic Amino Alcohols
ChemCatChem ( IF 3.8 ) Pub Date : 2020-01-20 , DOI: 10.1002/cctc.201901910
C Claaßen ₁ , K Mack _{1,

2} , D Rother _{1,

2}

Affiliation

Online analytics provides insights into the progress of an ongoing reaction without the need for extensive sampling and offline analysis. In this study, we investigated benchtop NMR as an online reaction monitoring tool for complex enzyme cascade reactions. Online NMR was used to monitor a two‐step cascade beginning with an aromatic aldehyde and leading to an aromatic amino alcohol as the final product, applying two different enzymes and a variety of co‐substrates and intermediates. Benchtop NMR enabled the concentration of the reaction components to be detected in buffered systems in the single‐digit mM range without using deuterated solvent. The concentrations determined via NMR were correlated with offline samples analyzed via uHPLC and displayed a good correlation between the two methods. In summary, benchtop NMR proved to be a sensitive, selective and reliable method for online reaction monitoring in (multi‐step) biosynthesis. In future, online analytic systems such as the benchtop NMR devices described might not only enable direct monitoring of the reaction, but may also form the basis for self‐regulation in biocatalytic reactions.

中文翻译：

用于在线监测芳香族氨基醇生物催化合成反应的台式核磁共振

在线分析可以深入了解正在进行的反应的进展情况，而无需进行大量采样和离线分析。在这项研究中，我们研究了台式核磁共振作为复杂酶级联反应的在线反应监测工具。在线核磁共振用于监测从芳香醛开始直至最终产物芳香氨基醇的两步级联，应用两种不同的酶和各种共底物和中间体。台式 NMR 能够在缓冲系统中检测单位数 mM 范围内的反应组分浓度，而无需使用氘代溶剂。通过 NMR 测定的浓度与通过 uHPLC 分析的离线样品相关，并显示两种方法之间具有良好的相关性。总之，台式核磁共振被证明是一种灵敏、选择性和可靠的在线反应监测（多步）生物合成方法。未来，在线分析系统（例如所描述的台式核磁共振设备）不仅可以直接监测反应，还可以形成生物催化反应自我调节的基础。

更新日期：2020-01-21

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