S-Adenosyl-l-Methionine Salvage Impacts Psilocybin Formation in "Magic" Mushrooms.,ChemBioChem

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S-Adenosyl-l-Methionine Salvage Impacts Psilocybin Formation in "Magic" Mushrooms.
ChemBioChem ( IF 2.6 ) Pub Date : 2020-01-10 , DOI: 10.1002/cbic.201900649
Richard Demmler ₁ , Janis Fricke ₁ , Sebastian Dörner ₁ , Markus Gressler ₁ , Dirk Hoffmeister ₁

Affiliation

Psychotropic Psilocybe mushrooms biosynthesize their principal natural product psilocybin in five steps, among them a phosphotransfer and two methyltransfer reactions, which consume one equivalent of 5'-adenosine triphosphate (ATP) and two equivalents of S-adenosyl-l-methionine (SAM). This short but co-substrate-intensive pathway requires nucleoside cofactor salvage to maintain high psilocybin production rates. We characterized the adenosine kinase (AdoK) and S-adenosyl-l-homocysteine (SAH) hydrolase (SahH) of Psilocybe cubensis. Both enzymes are directly or indirectly involved in regenerating SAM. qRT-PCR expression analysis revealed an induced expression of the genes in the fungal primordia and carpophores. A one-pot in vitro reaction with the N-methyltransferase PsiM of the psilocybin pathway demonstrates a concerted action with SahH to facilitate biosynthesis by removal of accumulating SAH.

中文翻译：

S-腺苷-l-甲硫氨酸回收影响“神奇”蘑菇中裸盖菇素的形成。

精神药裸盖菇通过五个步骤生物合成其主要天然产物裸盖菇素，其中包括磷酸转移和两个甲基转移反应，消耗一当量的 5'-三磷酸腺苷 (ATP) 和两当量的 S-腺苷-l-蛋氨酸 (SAM)。这种短但共底物密集的途径需要核苷辅因子挽救以维持裸盖菇素的高生产率。我们对古巴裸盖菇的腺苷激酶 (AdoK) 和 S-腺苷-L-同型半胱氨酸 (SAH) 水解酶 (SahH) 进行了表征。这两种酶都直接或间接参与 SAM 的再生。 qRT-PCR 表达分析揭示了真菌原基和果梗中基因的诱导表达。与裸盖菇素途径的 N-甲基转移酶 PsiM 进行的一锅体外反应表明，与 SahH 具有协同作用，通过去除累积的 SAH 来促进生物合成。

更新日期：2020-01-10

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