Neuronal cell cultures have been used as an essential tool for studying pathomechanisms of toxicity of chemotherapeutic drugs and to develop neuroprotective approaches. They offer the opportunity to dissect disease mechanisms and molecular pathways while allowing precise control of a variety of confounding factors of the physio-chemical environment. As such, a growing number of in vitro studies are published each year to decipher mechanisms of neurotoxicity of taxanes, vinca alcaloids, proteasome inhibitors and platin derivatives and/or to test neuroprotective strategies. Here, we provide a review of cell culture techniques and outcome measures that have been used in the past or are currently employed to model chemotherapy induced neuropathy in vitro. Furthermore, we discuss their advantages as well as their limitations and ways to enhance efficiency and reproducibility of cell culture studies in the field of toxic neuropathy.

中文翻译：

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在体外模拟化疗诱导的周围神经病变 (CIPN)：前景和局限性。

神经元细胞培养物已被用作研究化学治疗药物毒性的病理机制和开发神经保护方法的重要工具。它们提供了剖析疾病机制和分子途径的机会，同时允许精确控制物理化学环境的各种混杂因素。因此，每年都会发表越来越多的体外研究，以破译紫杉烷、长春花生物碱、蛋白酶体抑制剂和铂衍生物的神经毒性机制和/或测试神经保护策略。在这里，我们回顾了过去使用或目前用于模拟化疗诱导的体外神经病变的细胞培养技术和结果测量。此外，