The posterodorsal medial amygdala (MePD) has a high concentration of receptors for gonadal hormones, is a sexually dimorphic region and dynamically controls the reproductive behavior of both males and females. Neurotrophic factors can promote dendritic spine remodeling and change synaptic input strength in a region-specific manner. Here, we analyzed the gene and protein expression of brain-derived neurotrophic factor (BDNF), insulin-like growth factor-I (IGF-1), polysialylated neural cell adhesion molecule (PSA-NCAM) and Ephrin-A4 in the MePD of adult males and females in diestrus, proestrus and estrus using real-time qPCR and fluorescent immunohistochemistry. The first approach showed their amplification except for Igf1 and the latter revealed that BDNF, IGF-1, PSA-NCAM and Ephrin-A4 are expressed in the MePD of the adult rats. Protein expression of these neurotrophic factors showed no differences between groups. However, proestrus females displayed a higher number of labelled puncta than males for BDNF expression and diestrus females for IGF-1 expression. In conjunction, results indicate that IGF-1 might be released rather than synthetized in the MePD, and the expression of specific neurotrophic factors varies specifically during proestrus. The dynamic modulation of BDNF and IGF-1 during this cyclic phase is coincident with synaptic changes and spine density remodeling in the MePD, the disinhibition of gonadotrophin secretion for ovulation and the display of sexual behavior.

中文翻译：

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雄性和骑行雌性大鼠后内侧内侧杏仁核的神经营养因子。

后体内侧杏仁核（MePD）具有高浓度的性腺激素受体，是性二态性区域，可以动态控制雄性和雌性的生殖行为。神经营养因子可以促进树突棘重塑并以区域特定方式改变突触输入强度。在这里，我们分析了脑源性神经营养因子（BDNF），胰岛素样生长因子-I（IGF-1），多唾液酸化神经细胞粘附分子（PSA-NCAM）和Ephrin-A4在MePD中的基因和蛋白质表达。使用实时定量PCR和荧​​光免疫组化技术检测雌性，发情期和发情期的成年男性和女性。第一种方法显示了它们除Igf1以外的扩增，而第二种方法显示了BDNF，IGF-1，PSA-NCAM和Ephrin-A4在成年大鼠的MePD中表达。这些神经营养因子的蛋白表达在各组之间没有差异。然而，对于BDNF表达，雌性前驱体显示出比男性更高的标记点，而对于IGF-1表达，雌性雌性显示出比雌性雌性更高的数量。结合起来，结果表明IGF-1可能在MePD中释放而不是合成，并且特定的神经营养因子的表达在发情期特别地变化。BDNF和IGF-1在此循环阶段的动态调节与MePD中的突触变化和脊柱密度重塑，促性腺激素分泌排卵的抑制和性行为的出现相吻合。对于BDNF表达，雌性前驱体显示的标记点数高于男性，而对于IGF-1表达，雌性前驱体显示出的雌性雌蕊数量更多。结合起来，结果表明IGF-1可能在MePD中释放而不是合成，并且特定的神经营养因子的表达在发情期特别地变化。BDNF和IGF-1在此循环阶段的动态调节与MePD中的突触变化和脊柱密度重塑，促排卵促性腺激素分泌的抑制以及性行为的显示相吻合。对于BDNF表达，雌性前驱体显示的标记点数高于男性，而对于IGF-1表达，雌性前驱体显示出的雌性雌蕊数量更多。结合起来，结果表明IGF-1可能在MePD中释放而不是合成，并且特定的神经营养因子的表达在发情期特别地变化。BDNF和IGF-1在此循环阶段的动态调节与MePD中的突触变化和脊柱密度重塑，促排卵促性腺激素分泌的抑制以及性行为的显示相吻合。