Six-month-old (FS6) and 12-month-old (FS12) Thai fish sauces were separated by Sephadex-G25 desalting columns into 3 fractions, namely, F1 (peptides/Maillard reaction products), F2 (peptides/NaCl) and F3 (peptides/amino acids). The F3 fractions from both samples showed the highest chemical and cellular antioxidant activities and could inhibit ACE (P < 0.05). F1 and F2 showed mitogenic effects on HepG2 cells. F3 exhibited cytoprotective effects and inhibited cellular reactive oxygen species (ROS) up to 50 µg l-leucine equivalent/ml while demonstrating a cellular pro-oxidation effect at 100 µg l-leucine equivalent/ml. The F3 from FS12 (F3-12) induced upregulation of genes encoding antioxidant enzymes, particularly SOD1, at a higher level than that from FS6 (F3-6) (P < 0.05). Glu was the predominant free amino acid in both fish sauce samples, while Phe, Trp and Tyr were predominant in F3s. Peptide identification showed that F3-6 and F3-12 primarily contained di- and tripeptides originating from myosin, creatine kinase, titin, actin and troponin.

通过Sephadex-G25脱盐柱将6个月大（FS6）和12个月大（FS12）的泰国鱼露分为三部分，分别为F1（肽/美拉德反应产物），F2（肽/ NaCl）和F3（肽/氨基酸）。两个样品中的F3馏分均显示出最高的化学和细胞抗氧化活性，并能抑制ACE（P <0.05）。F1和F2对HepG2细胞显示有丝分裂作用。F3表现出细胞保护作用和抑制细胞的活性氧物质（ROS）高达50微克升-亮氨酸当量/ ml，而以100μg展示蜂窝亲氧化效果升-亮氨酸当量/毫升。FS12的F3（F3-12）诱导编码抗氧化酶（尤其是SOD1）的基因上调，高于FS6（F3-6）的水平（P <0.05）。在两个鱼露样品中，Glu是主要的游离氨基酸，而在F3中，Phe，Trp和Tyr是主要氨基酸。肽鉴定表明，F3-6和F3-12主要包含源自肌球蛋白，肌酸激酶，肌动蛋白，肌动蛋白和肌钙蛋白的二肽和三肽。