By combined use of traditional Chinese acupuncture Fe needle electrode and in-vivo electrochemistry, we achieve in-vivo H₂ generation in tumors in a controllable manner and exploited it for effective and green therapy of tumor for the first time. The cathodic acupuncture electrodes working under the applied voltage of ~ 3 volt (with minimal damage to the living body) undergo effectively electrochemical reactions in the acidic tumor area to produce sufficient H₂ locally, causing cancer cells to burst and die. Due to puncture positioning, acidic tumor microenvironment and gas diffusion effect, the developed H₂ generation electrochemotherapy (H₂-ECT) strategy enables precise and large-scale tumor therapy, as demonstrated by in vivo treatment of diseased mice (glioma and breast cancers). Such green H₂-ECT is simple, highly efficient and minimally invasive, requiring no expensive medical equipment or (nano)materials and medication, and is therefore very promising for potential clinic applications.

中文翻译：

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基于体内电化学的绿色、高效、精准的癌症氢疗法

通过结合中药针刺Fe针电极和体内电化学，我们实现了肿瘤体内H ₂的可控生成，并首次将其用于肿瘤的有效绿色治疗。阴极针灸电极在~3伏的外加电压下工作（对活体损伤最小）在酸性肿瘤区域进行有效的电化学反应，在局部产生足够的H ₂，导致癌细胞破裂和死亡。由于穿刺定位、酸性肿瘤微环境和气体扩散效应，开发的H ₂代电化学疗法（H ₂-ECT) 策略能够实现精确和大规模的肿瘤治疗，正如对患病小鼠（神经胶质瘤和乳腺癌）的体内治疗所证明的那样。这种绿色H ₂ -ECT简单、高效且微创，不需要昂贵的医疗设备或（纳米）材料和药物，因此在潜在的临床应用中非常有前景。