BACKGROUND To evaluate the impact of follicle-flushing during oocyte collection on embryo development potential retrospectively. METHODS A total of 1714 cases, including 133 who experienced retrieval difficulty (repeated follicle-flushing) on the day of oocyte retrieval (difficulty group) and the control 1581 cases (control group), were assessed in this retrospective study. The number of oocytes recovered, two pro-nuclei fertilization (2PN-fertilization), day 3 good-quality embryo and day 5/6 blastocyst utilization rates were compared between the difficulty group and control group correspondingly. Embryo implantation, clinical pregnancy and neonatal outcomes were further analyzed between the two groups in the fresh day- 3 embryo transfer cycles. RESULTS The number of oocytes recovered in the difficulty group (9.08 ± 4.65) were significantly reduced compared with the control group (12.13 ± 5.27),P < 0.001; The 2PN-fertilization, day 3 good-quality embryo and blastocyst utilization rates were significantly lower in the difficulty group compared with controls (71.7% vs. 75.7%; 52.7% vs. 56.5%; 31.9% vs. 37.0%, all P < 0.05). Embryo implantation in the difficulty group was 53.2%, which was lower than the control value of 58.7%, although not reaching statistical significance. The rate of fresh embryo transfer cycles in the difficulty group was lower than normal ones (51.88% vs. 61.99%, P = 0.026). The pregnancy and live birth rates were similar between the two groups. But the rate of spontaneous miscarriages of the difficulty group was higher than the control group, although not reaching statistical significance. The neonatal outcomes had no statistical difference between the two groups. CONCLUSIONS Oocyte retrieval difficulty, which include repeated flushing and the corresponded extending time required for oocyte recovery, significantly reduced day 3 good-quality embryo and blastocyst utilization rates of these patients. But the live birth rate had no difference between the difficulty group and the normal ones.

中文翻译：

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卵母细胞收集过程中的卵泡冲洗对体外受精的胚胎发育的影响。

背景技术回顾性评估卵母细胞收集过程中的卵泡冲洗对胚胎发育潜力的影响。方法这项回顾性研究共评估了1714例病例，包括133例在卵母细胞取回当天经历了取回困难（重复卵泡冲洗）（困难组）和对照组1581例（对照组）。分别比较困难组和对照组的卵母细胞数，两次原核受精（2PN受精），第3天优质胚胎和第5/6天胚泡利用率。在新鲜的第3天胚胎移植周期中，进一步分析了两组之间的胚胎着床，临床妊娠和新生儿结局。结果困难组的卵母细胞恢复数为（9.08±4）。65）与对照组相比明显降低（12.13±5.27），P <0.001; 与对照组相比，困难组的2PN受精，第3天优质胚胎和胚泡利用率明显低于对照组（分别为71.7％和75.7％； 52.7％和56.5％； 31.9％和37.0％，所有P < 0.05）。尽管没有达到统计学显着性，但困难组的胚胎植入率为53.2％，低于对照组的58.7％。困难组的新鲜胚胎移植周期率低于正常组（51.88％vs. 61.99％，P = 0.026）。两组的妊娠率和活产率相似。但是，尽管没有达到统计学意义，但困难组的自然流产率高于对照组。两组之间的新生儿结局无统计学差异。结论卵母细胞取回困难，包括反复潮红和卵母细胞恢复所需的相应延长时间，显着降低了这些患者的第3天优质胚胎和胚泡利用率。但是活产率在困难组和正常组之间没有差异。