BACKGROUND Pasteurella multocida is one of the important pathogens that infect rabbits, causing major economic losses in commercial rabbit farming. In this study, 205 P. multocida isolates recovered from lungs of dead rabbits with respiratory disease were defined by capsular serogroups, lipopolysaccharide (LPS) genotypes, multi-locus sequence types and screened virulence factors by using PCR assays, and tested antimicrobial susceptibility. RESULTS The 205 isolates were assigned into 2 capsular types, A and D, and 2 LPS genotypes, L3 and L6. When combining capsular types with LPS genotypes, 4 serotypes were detected. A:L3 (51.22%, 105/205) was the most predominant serotype, followed by A:L6 (24.88%, 51/205), D:L6 (19.02%, 39/205) and D:L3 (4.88%, 10/205). The 205 isolates were grouped into 3 sequence types, ST10, ST11 and ST12. ST12 (56.10%, 115/205) was the most prevalent sequence type, followed by ST10 (24.88%, 51/205) and ST11 (19.02%, 39/205). In the 205 isolates, virulence associated genes ptfA, fur, hgbB, ompA, ompH and oma87 were positive in the PCR screening, whereas the toxA and tbpA genes were negative. Notably, the 156 capsular serogroup A isolates carried the pmHAS gene. All the 205 isolates were susceptible to most of the used antibiotics, except for streptomycin, gentamycin, kanamycin and ceftriaxone, and the resistance rates of which were 27.80, 15.61, 9.27 and 2.44%, respectively. CONCLUSIONS This study, for the first time, described the prevalence and characteristics of P. multocida causing respiratory disease in rabbits in Fujian Province, which might be useful for tracking the epidemic strains and development of efficient vaccines and methods to prevent and control the pathogen.

中文翻译：

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从福建省死于呼吸系统疾病的家兔中分离出多杀性巴斯德氏菌的特征。

背景技术多杀巴斯德氏菌是感染兔子的重要病原体之一，在商业化兔子养殖中造成重大的经济损失。在这项研究中，通过包膜血清群，脂多糖（LPS）基因型，多位点序列类型和使用PCR测定筛选的毒力因子，确定了从患有呼吸系统疾病的死兔的肺中回收的205个P. 结果将205株分离株分为2种荚膜类型A和D，以及2种LPS基因型L3和L6。当结合荚膜类型和LPS基因型时，检测到4种血清型。A：L3（51.22％，105/205）是最主要的血清型，其次是A：L6（24.88％，51/205），D：L6（19.02％，39/205）和D：L3（4.88％， 10/205）。205个分离株分为3种序列类型：ST10，ST11和ST12。ST12（56.10％，115/205）是最普遍的序列类型，其次是ST10（24.88％，51/205）和ST11（19.02％，39/205）。在205株分离物中，毒力相关基因ptfA，fur，hgbB，ompA，ompH和oma87在PCR筛选中呈阳性，而toxA和tbpA基因呈阴性。值得注意的是，156个荚膜血清群A分离株带有pmHAS基因。除链霉素，庆大霉素，卡那霉素和头孢曲松外，所有205种分离株均对大多数使用的抗生素敏感，其耐药率分别为27.80、15.61、9.27和2.44％。结论本研究首次描述了福建省家兔多发性疟原虫引起呼吸系统疾病的流行情况和特征，