Background Allergy to Vespa affinis venom is common in the Asia Pacific region. Venom preparations for diagnosis are not commercially available for this species. Methods The prominent allergens in V. affinis venom were identifiedusing immunochemical methods. Use of ImmunoCAP of Vespula vulgaris crude venom/its components and a passive basophil activation test (BAT) in the diagnosis of patients who had anaphylaxis to V. affinis venom (n = 30) were also accessed. The IgE double-positivity rates (positive to both hornet and honeybee) in ImmunoCAP and the passive BAT were determined. Results High IgE reactivity was seen with the five allergens in V. affinis venom; 96% (29/30) for 34 and 24 kDa, 93% (28/30) for 45 kDa and 90% (27/30) reactivity for the 100 and 80 kDa respectively. IgE cross-reactivity was low with ImmunoCAP using V. vulgaris venom (43%; 13/30) and Ves v1 (3%; 1/30), but relatively high with Ves v5 (73%; 22/30). All patients (100%) were positive to V. affinis venom in passive BAT. In ImmunoCAP, a high double-positivity rate (76%; 23/30) was detected while no double-positivity was detected in passive BAT. Conclusions High IgE reactivity for five allergens of V. affinis points to the potential of using these allergens in component resolved diagnosis (CRD). The passive BAT has shown its importance as a promising diagnostic tool with high accuracy. It would be particularly useful in cases with doubtful double-positive results of other diagnostic tests.

中文翻译：

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Vespa affinis 毒液过敏的诊断：使用免疫化学方法和被动嗜碱性粒细胞活化试验。

背景 对 Vespa affinis 毒液的过敏在亚太地区很常见。用于诊断的毒液制剂无法用于该物种。方法采用免疫化学方法鉴定亲缘弧菌毒液中的主要过敏原。还访问了使用 Vespula vulgaris 粗毒液/其成分的 ImmunoCAP 和被动嗜碱性粒细胞活化试验 (BAT) 来诊断对 V. affinis 毒液 (n = 30) 有过敏反应的患者。测定了 ImmunoCAP 和被动 BAT 中的 IgE 双阳性率（对大黄蜂和蜜蜂均呈阳性）。结果 V. affinis 毒液中的五种过敏原具有高 IgE 反应性；34 和 24 kDa 的反应性为 96% (29/30)，45 kDa 的反应性为 93% (28/30)，100 和 80 kDa 的反应性分别为 90% (27/30)。使用 V 的 ImmunoCAP 的 IgE 交叉反应性较低。vulgaris 毒液 (43%; 13/30) 和 Ves v1 (3%; 1/30)，但相对较高的是 Ves v5 (73%; 22/30)。所有患者 (100%) 在被动 BAT 中对 V. affinis 毒液呈阳性。在 ImmunoCAP 中，检测到高双阳性率（76%；23/30），而在被动 BAT 中未检测到双阳性。结论 对五种亲缘弧菌过敏原的高 IgE 反应性表明这些过敏原在成分分离诊断 (CRD) 中的应用潜力。被动 BAT 已显示出其作为一种有前途的高精度诊断工具的重要性。在其他诊断测试的双阳性结果可疑的情况下，这将特别有用。23/30) 被检测到，而在被动 BAT 中未检测到双阳性。结论 对五种亲缘弧菌过敏原的高 IgE 反应性表明这些过敏原在成分分离诊断 (CRD) 中的应用潜力。被动 BAT 已显示出其作为一种有前途的高精度诊断工具的重要性。在其他诊断测试的双阳性结果可疑的情况下，这将特别有用。23/30) 被检测到，而在被动 BAT 中未检测到双阳性。结论 对五种亲缘弧菌过敏原的高 IgE 反应性表明这些过敏原在成分分离诊断 (CRD) 中的应用潜力。被动 BAT 已显示出其作为一种有前途的高精度诊断工具的重要性。在其他诊断测试的双阳性结果可疑的情况下，这将特别有用。