Nanoplastics as a potential environmental health factor: effects of polystyrene nanoparticles on human intestinal epithelial Caco-2 cells,Environmental Science: Nano

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Nanoplastics as a potential environmental health factor: effects of polystyrene nanoparticles on human intestinal epithelial Caco-2 cells
Environmental Science: Nano ( IF 5.8 ) Pub Date : 2019/12/05 , DOI: 10.1039/c9en00523d
Constanza Cortés _{1,

2,

3,

4,

5} , Josefa Domenech _{1,

2,

3,

4,

5} , Marcela Salazar _{1,

2,

3,

4,

5} , Susana Pastor _{1,

2,

3,

4,

5} , Ricard Marcos _{1,

2,

3,

4,

5} , Alba Hernández _{1,

2,

3,

4,

5}

Affiliation

The ubiquitous and increasing presence of micro-/nanoplastics (MNPLs) in our environment demands an urgent hazard assessment, in order to determine the potential risk they pose to human beings. Given the scarce information found in the literature regarding MNPL's effects over human cells, the aim of our work is to evaluate MNPL's ability to penetrate the cells, and their potential toxic/genotoxic effects. To this aim, we used polystyrene MNPLs, as they are a widespread model of synthetic polymer, using nanoparticles with (y-nPS) or without (nPS) a fluorescent label. The human colon adenocarcinoma Caco-2 cell line was used as the cellular target, as ingestion is one of the main entry routes of MNPLs. Different endpoints were analyzed as indicators of nanotoxicity, including cytotoxicity, ROS increase, genotoxicity, DNA oxidative damage and increase in the expression of stress-related genes.

中文翻译：

纳米塑料是潜在的环境健康因素：聚苯乙烯纳米颗粒对人肠上皮Caco-2细胞的影响

在我们的环境中无处不在且不断增加的微/纳米塑料（MNPL）要求进行紧急危害评估，以确定它们对人类构成的潜在风险。考虑到文献中关于MNPL对人类细胞的影响的信息稀少，我们的工作目标是评估MNPL穿透细胞的能力及其潜在的毒性/遗传毒性作用。为此，我们使用聚苯乙烯MNPL，因为它们是合成聚合物的广泛模型，使用具有（y-nPS）或不具有（nPS）荧光标记的纳米颗粒。人类结肠腺癌Caco-2细胞系被用作细胞靶标，因为摄入是MNPLs的主要进入途径之一。分析了不同的终点作为纳米毒性的指标，包括细胞毒性，ROS增加，遗传毒性，

更新日期：2020-02-13

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