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Sustained release of growth factors from photoactivated platelet rich plasma (PRP).
European Journal of Pharmaceutics and Biopharmaceutics ( IF 4.4 ) Pub Date : 2019-12-05 , DOI: 10.1016/j.ejpb.2019.11.011 Gülseren Irmak 1 , Tuğrul Tolga Demirtaş 1 , Menemşe Gümüşderelioğlu 2
European Journal of Pharmaceutics and Biopharmaceutics ( IF 4.4 ) Pub Date : 2019-12-05 , DOI: 10.1016/j.ejpb.2019.11.011 Gülseren Irmak 1 , Tuğrul Tolga Demirtaş 1 , Menemşe Gümüşderelioğlu 2
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The goal of this study is to specify the ability of polychromatic light source (PAC), providing effective wavelengths in the range of 600-1200 nm (near-infrared region, NIR), to activate human platelets in platelet-rich plasma (PRP) and to achieve sustained and controlled release of growth factors from photoactivated platelets. PRP was isolated from human blood and treated with PAC in different time intervals during 1, 5 and 10 min from 10 cm distance to the platelets. ATP secretion and then, calcium release from platelets significantly increased after light application. Photostimulation of platelets triggered lamellipodia extension, numerous filopodia formation, and platelet agglomeration as activation indicators. P-selectin expression was significantly increased after the application of PAC. In conclusion, PRP was successfully activated with PAC for 10 min and realized activation-dependent sustained growth factor release during 28 days. We proved that PAC which has a great potential of activation of PRP enables sustained growth factor release from PRP with a periodic use for therapeutic applications of PRP.
中文翻译：
从富含光活化血小板的血浆（PRP）中持续释放生长因子。
这项研究的目的是确定提供在600-1200 nm（近红外区域，NIR）范围内的有效波长的多色光源（PAC）激活富血小板血浆（PRP）中的人类血小板的能力。并实现从光活化血小板中持续释放和控制释放生长因子。从人血中分离出PRP，并在距血小板10厘米的距离分别为1、5和10分钟的不同时间间隔内，用PAC处理。光照后，ATP分泌增加，然后从血小板释放的钙明显增加。血小板的光刺激触发层状脂膜扩张，大量丝状伪足形成和血小板聚集作为激活指标。应用PAC后，P-选择素的表达显着增加。综上所述，PRP已成功用PAC激活10分钟，并在28天内实现了依赖激活的持续生长因子释放。我们证明了具有极大激活PRP潜力的PAC可以定期从PRP的治疗应用中使PRP持续释放生长因子。
更新日期：2019-12-05
中文翻译：
从富含光活化血小板的血浆（PRP）中持续释放生长因子。
这项研究的目的是确定提供在600-1200 nm（近红外区域，NIR）范围内的有效波长的多色光源（PAC）激活富血小板血浆（PRP）中的人类血小板的能力。并实现从光活化血小板中持续释放和控制释放生长因子。从人血中分离出PRP，并在距血小板10厘米的距离分别为1、5和10分钟的不同时间间隔内，用PAC处理。光照后，ATP分泌增加，然后从血小板释放的钙明显增加。血小板的光刺激触发层状脂膜扩张，大量丝状伪足形成和血小板聚集作为激活指标。应用PAC后，P-选择素的表达显着增加。综上所述，PRP已成功用PAC激活10分钟，并在28天内实现了依赖激活的持续生长因子释放。我们证明了具有极大激活PRP潜力的PAC可以定期从PRP的治疗应用中使PRP持续释放生长因子。
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