The decrease in killing sensitivity of the cell membrane to microsecond pulse electric fields (μs-PEFs) is ascribed mainly to the aberrant morphology of cancer cells, with clear statistical correlations observed between cell size and shape defects and the worsening of the electrical response to the PEF. In this paper, nanosecond pulsed electric fields (ns-PEFs) inducing the nucleus effect and μs-PEFs targeting the cell membrane were combined to enhance destruction of irregular cells. The fluorescence dissipation levels of the nuclear membrane and cell membrane exposed to the μs, ns, and ns + μs pulse protocols were measured and compared, and a dynamic electroporation model of irregular cells was established by the finite element software COMSOL. The results suggest that the cell membrane disruption induced by μs-PEFs is worse for extremely irregular cells and depends strongly on cellular morphology. However, the nuclear membrane disruption induced by ns-PEFs does not scale with irregularity, suggesting the use of a combination of ns-PEFs with μs-PEFs to target the nuclear and cell membranes. We demonstrate that ns + μs pulses can significantly enhance the fluorescence dissipation of the cell and nuclear membranes. Overall, our findings indicate that ns + μs pulses may be useful in the effective killing of irregular cells.

中文翻译：

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靶向细胞内消融的纳秒脉冲会增加具有不规则形态的肿瘤细胞的破坏。

细胞膜对微秒脉冲电场（μs-PEFs）的杀伤敏感性降低主要归因于癌细胞的异常形态，在细胞大小和形状缺陷之间以及与对细胞的电反应恶化之间观察到明显的统计相关性。 PEF。本文将诱导细胞核效应的纳秒脉冲电场（ns-PEFs）和靶向细胞膜的μs-PEFs相结合，以增强对不规则细胞的破坏。测量并比较了暴露于μs，ns和ns +μs脉冲协议下的核膜和细胞膜的荧光消散水平，并通过有限元软件COMSOL建立了不规则细胞的动态电穿孔模型。结果表明，由μs-PEFs诱导的细胞膜破裂对于极其不规则的细胞更为严重，并且强烈依赖于细胞的形态。但是，由ns-PEF诱导的核膜破坏并不会不规则地扩大规模，这表明使用ns-PEF和μs-PEF的组合来靶向核膜和细胞膜。我们证明ns +μs脉冲可以显着增强细胞和核膜的荧光消散。总体而言，我们的发现表明ns +μs脉冲可能对有效杀死不规则细胞有用。我们证明ns +μs脉冲可以显着增强细胞和核膜的荧光消散。总体而言，我们的发现表明ns +μs脉冲可能对有效杀死不规则细胞有用。我们证明ns +μs脉冲可以显着增强细胞和核膜的荧光消散。总体而言，我们的发现表明ns +μs脉冲可能对有效杀死不规则细胞有用。