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Immunochromatographic tests for the detection of microcystin-LR toxin in water and fish samples
Analytical Methods ( IF 2.7 ) Pub Date : 2019/12/04 , DOI: 10.1039/c9ay01970g Elena A. Zvereva 1, 2, 3, 4 , Olga D. Hendrickson 1, 2, 3, 4 , Anatoly V. Zherdev 1, 2, 3, 4 , Boris B. Dzantiev 1, 2, 3, 4
Analytical Methods ( IF 2.7 ) Pub Date : 2019/12/04 , DOI: 10.1039/c9ay01970g Elena A. Zvereva 1, 2, 3, 4 , Olga D. Hendrickson 1, 2, 3, 4 , Anatoly V. Zherdev 1, 2, 3, 4 , Boris B. Dzantiev 1, 2, 3, 4
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Microcystins are toxic microalgae metabolites that exert adverse effects on human and environmental health. Rapid and sensitive analytical techniques for their control are in increasing demand. Here, three immunochromatographic assay formats were developed and compared for the detection of microcystin-LR (MC-LR), the predominant microcystin variant. These assay formats incorporated either direct labeling with antibodies conjugated with gold nanoparticles or indirect labeling via anti-species antibodies or via the biotin–streptavidin interaction. The best sensitivity and stability were obtained with the indirect labeling formats. The limit of instrument detection of the indirect immunochromatographic assay was 0.2 ng mL−1, while the limit of visual detection was 1 ng mL−1. The latter value corresponds to the maximum permissible value of MC-LR in drinking water as determined by the World Health Organization. The developed test system was successfully applied to MC-LR detection in fish samples, which required brief sample preparation, and water samples, which did not require any sample preparation. The assay analysis time was 20 minutes. The rapidity and simplicity of the proposed immunochromatographic method suggest that it is an effective tool for the large-scale screening and control of microcystins.
中文翻译：
免疫色谱法检测水和鱼样品中的微囊藻毒素-LR毒素
微囊藻毒素是有毒的微藻代谢产物，会对人类和环境健康产生不利影响。控制它们的快速和灵敏的分析技术的需求日益增长。在这里，开发了三种免疫层析测定形式，并进行了比较，以检测微囊藻毒素-LR（MC-LR）（微囊藻毒素的主要变异体）。这些测定形式并入要么直接标记与金纳米颗粒或间接标记的抗体缀合经由抗-物种抗体或通过生物素-链霉亲和相互作用。使用间接标记格式可获得最佳的灵敏度和稳定性。间接免疫色谱分析的仪器检测极限为0.2 ng mL -1，而视觉检测的极限是1 ng mL -1。后一个值对应于世界卫生组织确定的饮用水中MC-LR的最大允许值。所开发的测试系统已成功应用于鱼样品中的MC-LR检测，这些样品需要简单的样品制备，水样中的样品无需任何样品制备。测定分析时间为20分钟。所提出的免疫层析方法的快速性和简便性表明，它是大规模筛选和控制微囊藻毒素的有效工具。
更新日期：2020-02-10
中文翻译：
免疫色谱法检测水和鱼样品中的微囊藻毒素-LR毒素
微囊藻毒素是有毒的微藻代谢产物，会对人类和环境健康产生不利影响。控制它们的快速和灵敏的分析技术的需求日益增长。在这里，开发了三种免疫层析测定形式，并进行了比较，以检测微囊藻毒素-LR（MC-LR）（微囊藻毒素的主要变异体）。这些测定形式并入要么直接标记与金纳米颗粒或间接标记的抗体缀合经由抗-物种抗体或通过生物素-链霉亲和相互作用。使用间接标记格式可获得最佳的灵敏度和稳定性。间接免疫色谱分析的仪器检测极限为0.2 ng mL -1，而视觉检测的极限是1 ng mL -1。后一个值对应于世界卫生组织确定的饮用水中MC-LR的最大允许值。所开发的测试系统已成功应用于鱼样品中的MC-LR检测，这些样品需要简单的样品制备，水样中的样品无需任何样品制备。测定分析时间为20分钟。所提出的免疫层析方法的快速性和简便性表明，它是大规模筛选和控制微囊藻毒素的有效工具。
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