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Controlled Electrodeposition of Au‐Copper Oxide Nanocomposite on a Renewable Carbon Ceramic Electrode for Sensitive Determination of NADH in Serum Samples
Electroanalysis ( IF 2.7 ) Pub Date : 2019-12-04 , DOI: 10.1002/elan.201900592 Rahim Mohammad‐Rezaei 1 , Mahdi Golmohammadpour 1
Electroanalysis ( IF 2.7 ) Pub Date : 2019-12-04 , DOI: 10.1002/elan.201900592 Rahim Mohammad‐Rezaei 1 , Mahdi Golmohammadpour 1
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In this study, a sensitive nicotinamide adenine dinucleotide (NADH) biosensor based on Au‐Copper oxide nanocomposite modified carbon ceramic electrode (Au−CuO/CCE) was introduced. The developed NADH biosensor was prepared by controlled electrodeposition of copper and Au nanoparticles on the surface of a renewable CCE and was turned to Au−CuO/CCE by cycling the potential in alkaline media. The prepared electrode was carefully characterized with scanning electron microscopy, X‐ray diffraction, atomic force microscopy and cyclic voltammetry techniques. According to scan rate study, surface coverage (Γ) of the fabricated Au−CuO/CCE was calculated to be 1.54×10−8 mol cm−2 which was 3 time more than CuO/CCE. The fabricated electrode is well stable which could be reliably utilized for the determination of NADH with amperometry technique over the concentration range of 1–29 μM with sensitivity and detection limit (S/N=3) of 0.1025 μA μM−1 and 0.09 μM respectively. The prepared biosensor was used for NADH determination in serum samples with fast response time and satisfactory analytical results.
中文翻译：
可再生碳陶瓷电极上可控电沉积金-铜氧化物纳米复合材料，用于灵敏测定血清样品中的NADH
在这项研究中，介绍了一种基于金铜氧化物纳米复合改性碳陶瓷电极（Au-CuO / CCE）的灵敏烟酰胺腺嘌呤二核苷酸（NADH）生物传感器。通过在可再生CCE表面上电沉积铜和Au纳米粒子，制备了开发的NADH生物传感器，并通过在碱性介质中循环电势将其转变为Au-CuO / CCE。使用扫描电子显微镜，X射线衍射，原子力显微镜和循环伏安法对制备的电极进行了仔细的表征。根据扫描速率研究，所制造的Au-CuO / CCE的表面覆盖率（Γ）计算为1.54×10 -8 mol cm -2是CuO / CCE的3倍。制成的电极非常稳定，可以通过安培法可靠地用于浓度范围为1–29μM的NADH测定，灵敏度和检测极限（S / N = 3）分别为0.1025μAμM -1和0.09μM 。制备的生物传感器用于血清样品中NADH的测定，具有快速的响应时间和令人满意的分析结果。
更新日期：2019-12-04
中文翻译：
可再生碳陶瓷电极上可控电沉积金-铜氧化物纳米复合材料，用于灵敏测定血清样品中的NADH
在这项研究中，介绍了一种基于金铜氧化物纳米复合改性碳陶瓷电极（Au-CuO / CCE）的灵敏烟酰胺腺嘌呤二核苷酸（NADH）生物传感器。通过在可再生CCE表面上电沉积铜和Au纳米粒子，制备了开发的NADH生物传感器，并通过在碱性介质中循环电势将其转变为Au-CuO / CCE。使用扫描电子显微镜，X射线衍射，原子力显微镜和循环伏安法对制备的电极进行了仔细的表征。根据扫描速率研究，所制造的Au-CuO / CCE的表面覆盖率（Γ）计算为1.54×10 -8 mol cm -2是CuO / CCE的3倍。制成的电极非常稳定，可以通过安培法可靠地用于浓度范围为1–29μM的NADH测定，灵敏度和检测极限（S / N = 3）分别为0.1025μAμM -1和0.09μM 。制备的生物传感器用于血清样品中NADH的测定，具有快速的响应时间和令人满意的分析结果。
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