Abstract Because various stimuli can affect living organisms, substances that response to stimuli, such as H2O2, have been studied for biomaterials. To synthesize a biocompatible H2O2-responsive ionic cross-linker, we synthesized a water-soluble H2O2-responsive diselenide compounds containing both sulfate end groups (diSe-SO4). The synthesis of diSe-SO4 was evaluated by 1H NMR and FT-IR. H2O2 responsiveness was evaluated by UV–vis spectra, FT-IR, and XPS. The optical density at 303 nm due to diselenide in diSe-SO4 was decreased in the H2O2 concentration increased. FT-IR and XPS results showed new peaks at 880 cm and 3500 cm−1, and at 63.0 eV, respectively, due to oxidized Se, which indicated the cleavage of the diselenide bond in diSe-SO4 as the H2O2 concentration increased. The ionic binding efficiency of diSe-SO4 to cationic materials was confirmed by measuring the zeta-potential difference (from –15.6 mV to 10.3 mV) before and after mixing with chitosan. MRC-5 cell viability and apoptosis in the presence of diSe-SO4 was evaluated using a CCK-8 assay and FACS, respectively. A high cell viability exceeding 95% was observed at 500 μM diSe-SO4 over 48 h of culture. In conclusion, the synthesized water-soluble diSe-SO4 provides a H2O2-responsive ionic cross-linking material with a high cell affinity.

中文翻译：

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作为 H2O2 响应离子交联剂的水溶性硫酸化二硒化物的合成和细胞亲和力

摘要 由于各种刺激都会影响生物体，因此对刺激做出反应的物质，如H2O2，已被研究作为生物材料。为了合成生物相容的 H2O2 响应离子交联剂，我们合成了一种水溶性的 H2O2 响应二硒化物，其中含有两个硫酸盐端基 (diSe-SO4)。diSe-SO4 的合成通过 1H NMR 和 FT-IR 进行评估。H2O2 响应性通过紫外-可见光谱、FT-IR 和 XPS 进行评估。由于 diSe-SO4 中的二硒化物，在 H2O2 浓度增加时，303 nm 处的光密度降低。FT-IR 和 XPS 结果分别在 880 cm 和 3500 cm-1 以及 63.0 eV 处出现新峰，这是由于氧化的 Se，这表明随着 H2O2 浓度的增加，diSe-SO4 中的二硒键断裂。通过测量与壳聚糖混合前后的 zeta 电位差（从 –15.6 mV 到 10.3 mV），确认了 diSe-SO4 与阳离子材料的离子结合效率。分别使用 CCK-8 测定和 FACS 评估在 diSe-SO4 存在下的 MRC-5 细胞活力和细胞凋亡。在 500 μM diSe-SO4 培养 48 小时内观察到超过 95% 的高细胞活力。总之，合成的水溶性 diSe-SO4 提供了一种具有高细胞亲和力的 H2O2 响应离子交联材料。