INTRODUCTION Food and dietary ingredients have significant effects on metabolism and health. OBJECTIVE To evaluate whether and how different diets affected the serum lipidomic profile of dogs. METHODS Sixteen healthy beagles were fed a commercial dry diet for 3 months (control diet). After an overnight fasting period, a blood sample was taken for serum lipidomic profile analysis, and each dog was then randomly assigned to one of two groups. Group 1 was fed a commercial diet (Diet 1) and group 2 was fed a self-made, balanced diet supplemented with linseed oil and salmon oil (Diet 2) for 3 months. After an overnight fasting period, a blood sample was taken from each dog. Serum cholesterol and triacylglycerol analyses were performed and the serum lipidomic profiles were analyzed using targeted liquid chromatography-mass spectrometry. RESULTS Dogs fed the supplemented self-made diet (Diet 2) had significantly higher omega-3 fatty acid-containing lipids species and significantly lower saturated and mono- and di-unsaturated lipid species. Concentrations of sphingosine 1-phosphate species S1P d16:1 and S1P d17:1 were significantly increased after feeding Diet 2. CONCLUSION This study found that different diets had significant effects on the dog's serum lipidomic profile. Therefore, in studies that include lipidomic analyses, diet should be included as a confounding factor.

中文翻译：

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接受不同饮食的健康比格犬的血清脂质组分析。

简介食品和饮食成分对新陈代谢和健康具有重要影响。目的评估不同饮食是否以及如何影响狗的血清脂质组学。方法对16只健康的小猎犬进行商业性干粮喂养3个月（对照饮食）。禁食过夜后，抽取血液样本进行血清脂质组学分析，然后将每只狗随机分为两组。第1组饲喂商业饮食（饮食1），第2组饲喂自制的均衡饮食，辅以亚麻子油和鲑鱼油（饮食2），为期3个月。禁食过夜后，从每只狗身上取血样。进行血清胆固醇和三酰基甘油分析，并使用靶向液相色谱-质谱法分析血清脂质组学。结果饲喂补充自制饮食（饮食2）的狗的含omega-3脂肪酸的脂质种类明显较高，饱和，单不饱和和双不饱和脂质种类均明显较低。喂食饮食2后，1-磷酸鞘氨醇物种S1P d16：1和S1P d17：1的浓度显着增加。结论该研究发现，不同饮食对狗的血清脂质组学有显着影响。因此，在包括脂质组学分析的研究中，应将饮食作为混杂因素。喂食饮食2后1明显增加。结论结论本研究发现不同饮食对狗的血清脂质组学有显着影响。因此，在包括脂质组学分析的研究中，应将饮食作为混杂因素。喂食饮食2后1明显增加。结论结论本研究发现不同饮食对狗的血清脂质组学有显着影响。因此，在包括脂质组学分析的研究中，应将饮食作为混杂因素。