In this work, the metal oxide and biopolymer nanocomposites on multiwalled carbon nanotubes (MWCNT) were prepared using a simple sonochemical method. The hexagonal nanorods of zinc oxide (ZnO NR) were synthesized by probe sonication (frequency = 20 kHz, amplitude = 50) method and were integrated on ultrasonically functionalized MWCNT-cellulose nanocrystals (MWCNT-CNC) for the first time. The stable hemin bio-composites also were prepared using the bath sonication (37 kHz of frequency, 150 W of power) method, and was used for the selective and ultrasensitive electrochemical detection of H2O2. The UV-Vis spectroscopy studies confirmed the presence of native hemin on MWCNT-CNC/ZnO NR nanocomposite. Cyclic voltammetry studies revealed that an enhanced redox electrochemical behaviour of hemin was observed on hemin immobilised MWCNT-CNC/ZnO NR nanocomposite than that of other hemin modified electrodes. Also, the MWCNT-CNC/ZnO NR/hemin modified SPCE showed 2.3 folds higher electrocatalytic activity with a lower reduction potential (-0.2 V) towards H2O2 than that of other investigated hemin modified electrodes including hemin/MWCNT and hemin/CNC-ZnO. The fabricated biosensor displayed a stable amperometric response (-0.2 V vs Ag/AgCl) in the linear concentration of H2O2 ranging up to 4183.3 µM with a lower detection limit of 4.0 nM.

中文翻译：

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声化学合成和氧化锌在血红素介导的多壁碳纳米管-纤维素纳米复合材料上的锚固，用于超灵敏的H2O2生物传感。

在这项工作中，使用简单的声化学方法制备了多壁碳纳米管（MWCNT）上的金属氧化物和生物聚合物纳米复合材料。通过探针超声处理（频率= 20 kHz，振幅= 50）方法合成了六边形的氧化锌纳米棒（ZnO NR），并首次将其集成在超声功能化的MWCNT-纤维素纳米晶体（MWCNT-CNC）上。还使用浴超声处理（频率为37 kHz，功率为150 W）制备稳定的血红素生物复合物，并将其用于H2O2的选择性和超灵敏电化学检测。紫外可见光谱研究证实了天然血红素存在于MWCNT-CNC / ZnO NR纳米复合材料上。循环伏安法研究表明，在固定有血红素的MWCNT-CNC / ZnO NR纳米复合材料上，血红素的氧化还原电化学行为比其他由血红素修饰的电极观察到的增强。此外，MWCNT-CNC / ZnO NR / hemin修饰的SPCE的电催化活性高2.3倍，对H2O2的还原电位较低（-0.2 V），是其他研究的hemin修饰电极（包括hemin / MWCNT和hemin / CNC-ZnO）的2.3倍。所制造的生物传感器在线性浓度为4183.3 µM的H2O2中显示出稳定的安培响应（-0.2 V对Ag / AgCl），检测下限为4.0 nM。与其他研究的血红素修饰电极（包括血红素/ MWCNT和血红素/ CNC-ZnO）相比，向H2O2迁移2 V）。所制造的生物传感器在线性浓度为4183.3 µM的H2O2中显示出稳定的安培响应（-0.2 V对Ag / AgCl），检测下限为4.0 nM。与其他研究的血红素修饰电极（包括血红素/ MWCNT和血红素/ CNC-ZnO）相比，向H2O2迁移2 V）。所制造的生物传感器在线性浓度为4183.3 µM的H2O2中显示出稳定的安培响应（-0.2 V对Ag / AgCl），检测下限为4.0 nM。