Next-generation sequencing (NGS) can enable a more effective response to a wide range of communicable disease threats, such as influenza, which is one of the leading causes of human morbidity and mortality worldwide. After vaccination, antivirals are the second line of defense against influenza. The use of currently available antivirals can lead to antiviral resistance mutations in the entire influenza genome. Therefore, the methods to detect these mutations should be developed and implemented. In this Opinion, we assess how NGS could be implemented to detect drug resistance mutations in clinical influenza virus isolates.

中文翻译：

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下一代测序：在流感中抗病毒耐药性监测方面大开眼界

下一代测序（NGS）可以对多种传染病威胁（例如流感）做出更有效的反应，而流感是全世界人类发病和死亡的主要原因之一。接种疫苗后，抗病毒药是抵抗流感的第二道防线。使用当前可用的抗病毒药可导致整个流感基因组中的抗病毒耐药性突变。因此，应该开发和实施检测这些突变的方法。在本意见中，我们评估了如何实施NGS来检测临床流感病毒分离株中的耐药性突变。