With the characteristics of intrinsically free from photobleaching, blinking, and self-quenching, Raman microscopy has become a powerful platform in detecting molecular vibrations for diverse applications. In particular, Raman spectroscopy has proven very useful for bioanalysis but suffers from several challenges such as poor sensitivity, spectral overlapping, and background interference. In this review, we would like to introduce two kinds of high-fidelity imaging strategies, including background-free surface-enhanced Raman scattering (SERS) and stimulated Raman scattering (SRS) that are capable of significantly enhancing detection sensitivity and spectral resolution while minimizing background interference simultaneously. Recent advances in background-free SERS and SRS and their applications in biological detection and cellular imaging are summarized and discussed.

具有固有的免于光漂白，眨眼和自猝灭的特性，拉曼显微镜已成为检测分子振动以用于各种应用的强大平台。尤其是，拉曼光谱已被证明对生物分析非常有用，但存在一些挑战，例如灵敏度差，光谱重叠和背景干扰。在这篇综述中，我们想介绍两种高保真成像策略，包括无背景表面增强拉曼散射（SERS）和受激拉曼散射（SRS），它们能够显着提高检测灵敏度和光谱分辨率，同时将其最小化。同时发生背景干扰。