To date, studies on the application of loop-mediated isothermal amplification (LAMP) in the detection of genetically modified organisms (GMOs) are stably increasing and demonstrates LAMP is a potential and promising method for on spot identification of GMOs. However, little information is known for detection of GM potato events by LAMP. In this report, we developed an optimized and visual LAMP assay with high specificity and sensitivity to rapidly amplify genomic DNA of potato EH92-527-1 within 45 min. The limit of detection of LAMP in our study is 10-fold higher than the conventional PCR. Furthermore, LAMP products can be directly observed via naked eyes by addition of SYBR Green I without gel electrophoresis analysis and PCR-based equipment. Therefore, the LAMP assay developed in this paper provides an efficient, convenient and cost-effective tool for the detection of GM potato EH92-527-1.

中文翻译：

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通过环介导的等温扩增方法对转基因马铃薯EH92-527-1进行可视，灵敏和快速的事件特异性检测。

迄今为止，关于环介导的等温扩增（LAMP）在检测转基因生物（GMO）中的应用的研究正在稳步增加，并证明了LAMP是现场鉴定GMO的潜在和有前途的方法。但是，关于LAMP检测转基因马铃薯事件的信息知之甚少。在本报告中，我们开发了一种优化的可视化LAMP分析方法，具有很高的特异性和敏感性，可在45分钟内快速扩增马铃薯EH92-527-1的基因组DNA。在我们的研究中，LAMP的检出限比常规PCR高10倍。此外，无需添加凝胶电泳分析和基于PCR的设备，即可通过添加SYBR Green I肉眼直接观察LAMP产品。因此，本文开发的LAMP分析提供了一种有效的，