Large numbers of miRNAs are found in biofluid exosomes. We isolated ~50-200 nm diameter exosomes from four types of porcine biofluid (urine, plasma, semen, and bile) using serial centrifugation and ultracentrifugation procedures. A total of 42.15 M raw data were generated from four small RNA libraries. This produced 40.17 M map-able sequences, of which we identified 204 conserved miRNAs, and 190 novel candidate miRNAs. Furthermore, we identified 34 miRNAs specifically expressed in only one library, all with well-characterized immune-related functions. A set of five universally abundant miRNAs (miR-148a-3p, miR-21-5p, let-7f-5p, let-7i-5p, and miR-99a-5p) across all four biofluids was also found. Function enrichment analysis revealed that the target genes of the five ubiquitous miRNAs are primarily involved in immune and RNA metabolic processes. In summary, our findings suggest that porcine biofluid exosomes contain a large number of miRNAs, many of which may be crucial regulators of the immune system.

中文翻译：

---

高通量测序揭示了与猪免疫相关的生物流体外泌体miRNA。

在生物流体外泌体中发现了大量的miRNA。我们使用连续离心和超速离心程序从四种类型的猪生物流体（尿液，血浆，精液和胆汁）中分离出直径约50-200 nm的外泌体。从四个小型RNA文库中生成了总计42.15 M的原始数据。这产生了40.17 M的可定位序列，其中我们鉴定了204个保守的miRNA和190个新的候选miRNA。此外，我们鉴定出仅在一个文库中特异性表达的34个miRNA，所有这些miRNA都具有很好的免疫相关功能。还发现了在所有四个生物流体中的一组五个通用富裕的miRNA（miR-148a-3p，miR-21-5p，let-7f-5p，let-7i-5p和miR-99a-5p）。功能富集分析表明，五个普遍存在的miRNA的靶基因主要参与免疫和RNA代谢过程。