We previously reported that MDA-MB-231 and MCF-7 cells, which are breast cancer cell lines and have cancer and cancer-initiating cells (CICs), were killed following normothermic microwave irradiation in which the cellular temperature was maintained at 37°C. In this study, we investigated the percentages of live or dead cells among CD44+/CD24- cells, which were defined as CICs among MDA-MB-231 and MCF-7 cells, and other types of cells in response to microwave irradiation. CD44+/CD24- cells among MDA-MB-231 cells were killed, thereby decreasing the number of cells, whereas the number of live CD44+/CD24- MCF-7 cells was increased following microwave irradiation. Moreover, adhesion, invasion, and migration were decreased in MDA-MB-231 cells, and the activation of matrix metalloproteinase-2 (MMP-2) in MDA-MB-231 cells was increased following microwave irradiation. These decreased cell activities might have been caused by MMP-2 activation and population changes in CD44+/CD24- in MDA-MB-231 cells.Abbreviations: APC: allophecocyanin; CBB: coomassie Brilliant Blue; CD: cluster of differentiation; CICs: cancer-initiating cells; FACS: fluorescence-activated cell sorting; FBS: fetal bovine serum; FITC: fluorescein isothiocyanate; FTDT: finite-difference time domain; HER2: human epidermal growth factor receptor type 2; PI: propidium iodide.

中文翻译：

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常温微波辐射对乳腺癌MDA-MB-231和MCF-7细胞系CD44 + / CD24‒的影响。

我们之前曾报道过，在常温微波辐射下，将乳腺癌细胞系MDA-MB-231和MCF-7细胞保持在37°C的温度后，它们被杀死，这些细胞具有癌细胞和癌起始细胞（CIC）。 。在这项研究中，我们调查了CD44 + / CD24-细胞中活细胞或死亡细胞的百分比，这些细胞被定义为MDA-MB-231和MCF-7细胞以及其他类型的细胞对微波辐射的CIC。MDA-MB-231细胞中的CD44 + / CD24-细胞被杀死，从而减少了细胞数量，而微波照射后，活CD44 + / CD24-MCF-7细胞的数量增加了。此外，MDA-MB-231细胞的黏附，侵袭和迁移减少，微波照射后，MDA-MB-231细胞中基质金属蛋白酶2（MMP-2）的激活增加。这些降低的细胞活性可能是由于MDA-2活化和MDA-MB-231细胞中CD44 + / CD24-的种群变化所致。CBB：考马斯亮蓝；CD：分化集群；CIC：癌症引发细胞；FACS：荧光激活细胞分选；FBS：胎牛血清；FITC：异硫氰酸荧光素；FTDT：时域有限差分；HER2：人类表皮生长因子受体2型；PI：碘化丙锭。癌症起始细胞；FACS：荧光激活细胞分选；FBS：胎牛血清；FITC：异硫氰酸荧光素；FTDT：时域有限差分；HER2：人类表皮生长因子受体2型；PI：碘化丙锭。癌症起始细胞；FACS：荧光激活细胞分选；FBS：胎牛血清；FITC：异硫氰酸荧光素；FTDT：时域有限差分；HER2：人类表皮生长因子受体2型；PI：碘化丙锭。