Analytical performance and tissue interactions of nitric oxide (NO)-releasing continuous glucose sensors were evaluated over a 28 d study in a diabetic swine model. Interstitial glucose was detected using an implanted needle-type amperometric glucose sensor. Two NO-release durations from the sensor surface were achieved by doping the membranes with nonporous (14 d release) or porous (30 d release) S-nitrosothiol-functionalized silica nanoparticles. Numerical and clinical accuracy of the sensors were assessed at time points (1, 7, 14, 21, and 28 d) following implantation. Nitric oxide-releasing sensors demonstrated accurate glucose detection over a time period directly correlated with the active release of NO. Silica particle-doped sensors that released NO for 30 d showed standard-compliant accuracy (i.e., mean absolute relative difference ≤ 15%) for >3 weeks post-implantation. Histological staining for inflammatory biomarkers suggested that the observed performance improvement was the result of decreased inflammatory cell count and a lower density collagen capsule.

中文翻译：

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通过延长一氧化氮释放的长期准确连续葡萄糖生物传感器。

在糖尿病猪模型中的 28 天研究中评估了释放一氧化氮 (NO) 的连续葡萄糖传感器的分析性能和组织相互作用。使用植入的针型电流型葡萄糖传感器检测间质葡萄糖。通过用无孔（14 天释放）或多孔（30 天释放）S-亚硝基硫醇官能化二氧化硅纳米粒子掺杂膜，实现了传感器表面的两个 NO 释放持续时间。在植入后的时间点（1、7、14、21 和 28 天）评估传感器的数值和临床准确性。一氧化氮释放传感器在与 NO 的主动释放直接相关的一段时间内表现出准确的葡萄糖检测。释放 NO 30 天的二氧化硅颗粒掺杂传感器显示出符合标准的精度（即，植入后 > 3 周的平均绝对相对差异 ≤ 15%）。炎症生物标志物的组织学染色表明，观察到的性能改善是炎症细胞计数减少和胶原蛋白胶囊密度降低的结果。