Step toward Roadside Sensing: Noninvasive Detection of a THC Metabolite from the Sweat Content of Fingerprints.,ACS Sensors

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Step toward Roadside Sensing: Noninvasive Detection of a THC Metabolite from the Sweat Content of Fingerprints.
ACS Sensors ( IF 8.2 ) Pub Date : 2019-12-17 , DOI: 10.1021/acssensors.9b02020
Erica Brunelle ₁ , Brenna Thibodeau ₁ , Alyssa Shoemaker ₁ , Jan Halámek ₁

Affiliation

The sudden increase in states legalizing marijuana has forced law enforcement into a situation where the use and consumption are legal, but there are no limitations for what is acceptable for driving or operating machinery. Using ultraviolet-visible (UV-vis) spectroscopy, fingerprints from volunteers who had used marijuana were analyzed via a competitive immunoassay for the detection of Δ9-tetrahydrocannabinol (Δ9-THC), the main psychoactive component of marijuana, and 11-nor-9-carboxy-THC (THC-COOH), one of the main metabolites produced in the body following the use/consumption of THC-related products. In this research, the THC-COOH metabolite and the enzyme-labeled conjugate compete against each other as the antigens for the system. The antibody used in this assay has a greater affinity for the metabolite; so, as its concentration increases, the absorbance of the system decreases due to less binding of the enzyme-labeled conjugate.

中文翻译：

迈向路边传感：从指纹的汗液含量中无创检测THC代谢物。

大麻合法化的州的激增迫使执法部门进入了合法使用和消费的情况，但是对于驱动或操作机械的可接受性没有任何限制。使用紫外可见（UV-vis）光谱，通过竞争性免疫分析法分析了使用大麻的志愿者的指纹，以检测大麻的主要精神活性成分Δ9-四氢大麻酚（Δ9-THC）和11-nor-9 -羧基-四氢大麻酚（THC-COOH），是使用/消费与四氢大麻酚相关的产品后在体内产生的主要代谢产物之一。在这项研究中，THC-COOH代谢产物和酶标记的缀合物彼此竞争，成为系统的抗原。此测定法中使用的抗体对代谢物具有更高的亲和力；因此，随着浓度的增加，

更新日期：2019-12-18

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