The objective of this work was to separate, identify and assess antioxidant peptides from the simulated gastrointestinal (GI) digestion of crucian carp (Carassius auratus) cooking juice (CCCJ), which has been previously found with this activity. The CCCJ after simulated GI digestion treatment was separated gradually by ultrafiltration and RP-HPLC. Five novel antioxidant peptides with 10-13 amino acid residues were identified by UPLC-MS/MS. Their in silico assessments showed amphiphilic nature, good sensory quality and different target sites in the human body. Meanwhile, their three-dimensional structure predictions exhibited at least one β-turn, β-sheet and/or α-helix with partial hydrophobic and/or net-charged residues exposed to the external medium, which was good evidence for high antioxidant activity. Ultimately, four novel peptides with high antioxidant activity were found, among which IREADIDGDGQVN (1401 Da), PEILPDGDHD (1107 Da) and ASDEQDSVRL (1119 Da) exerted the highest DPPH radical scavenging activity with IC50 of 1.78, 1.18 and 1.45 mM, respectively, while APLEEPSSPH (1063 Da) showed the highest Fe2+ chelating ability with IC50 of 0.09 mM. This work could help understand the mechanism of CCCJ on human health promotion and improve the economic value of the crucian carp processing industry.

中文翻译：

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通过UPLC-MS / MS和计算机分析，从模拟胃肠道消化中释放的cru鱼烹饪汁中的新型抗氧化剂肽的鉴定和表征。

这项工作的目的是从of鱼（Carassius auratus）蒸煮汁（CCCJ）的模拟胃肠道（GI）消化物中分离，鉴定和评估抗氧化肽，此前已发现这种活性。通过超滤和RP-HPLC逐步分离模拟GI消化处理后的CCCJ。通过UPLC-MS / MS鉴定了五个具有10-13个氨基酸残基的新型抗氧化剂肽。他们的计算机评估显示出两亲性，良好的感官质量以及人体中不同的目标部位。同时，他们的三维结构预测显示至少一个β-转角，β-折叠和/或α-螺旋，部分疏水和/或净电荷残基暴露于外部介质，这是高抗氧化活性的良好证据。最终，发现了四个具有高抗氧化活性的新型肽，其中IREADIDGDGQVN（1401 Da），PEILPDGDHD（1107 Da）和ASDEQDSVRL（1119 Da）发挥了最高的DPPH自由基清除活性，IC50分别为1.78、1.18和1.45 mM，而APLEEPSSPH Fe（1063 Da）具有最高的Fe2 +螯合能力，IC50为0.09 mM。这项工作可以帮助了解CCCJ促进人类健康的机制，并提高the鱼加工业的经济价值。