Colibacillosis is a major disease affecting poultry leads to high morbidity and mortality which causing tremendous economic losses worldwide. These economic disparities are amplified among low and middle-income where sanitation and hygiene are challenged by the increasing demand for quality sources of animal protein. With a view to investigating the prevalence of virulence genes and QACs resistance genes as well as monitoring the antibiogram of E. coli strains, a total of 368 specimens were collected from diseased broiler chickens (n = 226) and environmental sources (n = 142) at large-scale poultry farms in Ismailia Governorate, Egypt. The bacteriological examination proved that E. coli prevalence was 26.76% and 50.44% in the farm environment and diseased broilers, respectively. In tandem, the isolated E. coli strains were serogrouped, determining the most common serotypes were O78, O1:H7, O91:H21 and O126. Isolates were tested for antimicrobial susceptibility against 12 antibiotics, screened for 4 virulence genes (iss, papC, eaeA, and cfaI), and screened for 3 QACs resistance genes (qacEΔ1, qacA/B, and qacC/D). All the tested strains were positive for iss and papC genes, only 20.3% of the tested strains were positive for eaeA gene, moreover, the examined strains were negative to CFAI gene. Furthermore, all the tested strains were positive for qacEΔ1, qacA/B, and qacC/D genes. In conclusion; virulence genes (iss, papC) as well as QACs resistance genes are common in avian Pathogenic E. coli and environmental strains and are mainly associated with multi-drug resistance phenomena.

中文翻译：

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从环境和禽源分离的大肠杆菌中，多药耐药性（MDR）的发生以及毒力基因和QACs耐药基因的普遍性。

大肠杆菌感染是影响家禽的主要疾病，会导致高发病率和高死亡率，从而在全世界范围内造成巨大的经济损失。这些经济差异在中，低收入人群中加剧，那里对卫生和卫生的要求越来越高，这是对动物蛋白优质来源的需求日益增加。为了调查毒力基因和QACs抗性基因的流行率并监测大肠杆菌菌株的抗菌谱，从患病的肉鸡（n = 226）和环境来源（n = 142）中总共收集了368个标本在埃及伊斯玛利亚省的大型家禽农场。细菌学检查证明大肠杆菌农场环境和患病肉鸡的流行率分别为26.76％和50.44％。依次对分离的大肠杆菌菌株进行血清分组，确定最常见的血清型为O78，O1：H7，O91：H21和O126。测试了分离物对12种抗生素的抗药性，筛选了4种毒力基因（iss，papC，eaeA和cfaI），并筛选了3种QACs耐药基因（qacEΔ1，qacA / B和qacC / D）。所有被测菌株均对iss和papC基因呈阳性，仅20.3％的被测菌株对eaeA基因呈阳性，而且，所测菌株对CFAI呈阴性。基因。此外，所有测试的菌株均对qacEΔ1，qacA / B和qacC / D基因呈阳性。综上所述; 毒力基因（iss，papC）以及QACs抗性基因在禽致病性大肠杆菌和环境菌株中很常见，并且主要与多重耐药性现象相关。