The TaqMan-based quantitative Polymerase Chain Reaction (qPCR) method and the Plate Count (PC) method are both used in combination with primary and secondary mathematical modeling, to describe the growth curves of Leuconostoc mesenteroides and Weissella viridescens in vacuum-packaged meat products during storage under different isothermal conditions. Vacuum-Packaged Morcilla (VPM), a typical cooked blood sausage, is used as a representative meat product, with the aim of improving shelf-life prediction methods for those sorts of meat products. The standard curves constructed by qPCR showed good linearity between the cycle threshold (C_T) and log₁₀ CFU/g, demonstrating the high precision and the reproducible results of the qPCR method. The curves were used for the quantification of L. mesenteroides and W. viridescens in artificially inoculated VPM samples under isothermal storage (5, 8, 13 and 18 °C). Primally, both the qPCR and the PC methods were compared, and a linear regression analysis demonstrated a statistically significant linear correlation between the methods. Secondly, the Baranyi and Roberts model was fitted to the growth curve data to estimate the kinetic parameters of L. mesenteroides and W. viridescens under isothermal conditions, and secondary models were used to establish the dependence of the maximum specific growth rate on the temperature. The results proved that primary and secondary models were adequate for describing the growth curves of both methods in relation to both bacteria. In conclusion, the results of all the experiments proved that the qPCR method in combination with the PC method can be used to construct microbial growth kinetics and that primary and secondary mathematical modeling can be successfully applied to describe the growth of L. mesenteroides and W. viridescens in vacuum-packaged morcilla and, by extension, other cooked meat products with similar characteristics.

基于TaqMan的定量聚合酶链反应（qPCR）方法和平板计数（PC）方法均与一级和二级数学模型结合使用，以描述间肠隐球菌和Weissella viridescens在真空包装肉制品中的生长曲线。在不同的恒温条件下储存。真空包装的羊肚菌（VPM）是一种典型的煮熟的血肠，被用作代表性的肉制品，目的是改善这些肉制品的保质期预测方法。通过qPCR构建的标准曲线在循环阈值（C _T）和log ₁₀之间显示出良好的线性CFU / g，证明了qPCR方法的高精度和可重现的结果。该曲线用于在等温储存（5、8、13和18°C）下人工接种的VPM样品中的肠膜劳德氏菌和杀虫剂W. viridescens的定量。最初，比较了qPCR和PC方法，并且线性回归分析证明了这两种方法之间具有统计学意义的线性相关性。其次，Baranyi和罗伯茨模型拟合的生长曲线数据来估计的动力学参数L.肠系膜和W. viridescens在等温条件下，使用二级模型建立最大比生长速率对温度的依赖性。结果证明，一级和二级模型足以描述两种方法相对于两种细菌的​​生长曲线。总之，所有实验的结果证明，qPCR方法与PC方法的结合可用于构建微生物生长动力学，并且一级和二级数学模型可成功地用于描述中肠梭状芽孢杆菌和W.的生长。真空包装的莫尔西拉干酪中的viridescens，以及延伸到其他具有类似特征的熟肉产品中的viridescens。