Fluorescence imaging of intracellular active molecules plays an important role in understanding the occurrence and development of diseases. Resonance energy transfer (RET) has been recognized as a powerful technology in the design of fluorescence probes for biological imaging. Here, we summarize the novel approaches to fabrication of RET-based fluorescent probes systems such as the organic molecular fluorescent probe, nano fluorescent probe, protein probe and their recent applications in cellular fluorescence imaging.

中文翻译：

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基于共振能量转移的细胞荧光成像

细胞内活性分子的荧光成像在理解疾病的发生和发展中起着重要的作用。在设计用于生物成像的荧光探针时，共振能量转移（RET）已被认为是一项强大的技术。在这里，我们总结了基于RET的荧光探针系统（如有机分子荧光探针，纳米荧光探针，蛋白质探针）及其在细胞荧光成像中的最新应用的新型制备方法。