Thus study found the temporal and spatial relationship between production of aliphatic glucosinolate compounds and the expression profile of glucosinolate-related genes during growth and development in radish, Chinese cabbage, and their intergeneric hybrid baemoochae plants. Glucosinolates (GSLs) are one of major bioactive compounds in Brassicaceae plants. GSLs play a role in defense against microbes as well as chemo-preventative activity against cancer, which draw attentions from plant scientists. We investigated the temporal relationship between production of aliphatic Glucosinolate (GSLs) compounds and the expression profile of GSL related genes during growth and development in radish, Chinese cabbage, and their intergeneric hybrid, baemoochae. Over the complete life cycle, Glucoraphasatin (GRH) and glucoraphanin (GRE) predominated in radish, whereas gluconapin (GNP), glucobrassicanapin (GBN), and glucoraphanin (GRA) abounded in Chinese cabbage. Baemoochae contained intermediate levels of all GSLs studied, indicating inheritance from both radish and Chinese cabbage. Expression patterns of BCAT4, CYP79F1, CYP83A1, UGT74B1, GRS1, FMOgs-ox1, and AOP2 genes showed a correlation to their corresponding encoded proteins in radish, Chinese cabbage, and baemoochae. Interestingly, there is a sharp change in gene expression pattern involved in side chain modification, particularly GRS1, FMOgs-ox1, and AOP2, among these plants during the vegetative and reproductive stage. For instance, the GRS1 was strongly expressed during leaf development, while both of FMOgs-ox1 and AOP2 was manifested high in floral tissues. Furthermore, expression of GRS1 gene which is responsible for GRH production was predominantly expressed in leaf tissues of radish and baemoochae, whereas it was only slightly detected in Chinese cabbage root tissue, explaining why radish has an abundance of GRH compared to other Brassica plants. Altogether, our comprehensive and comparative data proved that aliphatic GSLs biosynthesis is dynamically and precisely regulated in a tissue- and development-dependent manner in Brassicaceae family members.

中文翻译：

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在发育过程中参与属间 Baemoochae（十字花科）及其亲本中脂肪族硫代葡萄糖苷生物合成的主要基因的差异表达。

因此，研究发现了萝卜、大白菜及其属间杂种白菜植物生长发育过程中脂肪族硫代葡萄糖苷化合物的产生与硫代葡萄糖苷相关基因表达谱的时空关系。硫代葡萄糖苷 (GSL) 是十字花科植物中的主要生物活性化合物之一。GSL在防御微生物和化学预防癌症方面发挥作用，引起了植物科学家的关注。我们研究了萝卜、大白菜及其属间杂种白菜的生长发育过程中脂肪族硫代葡萄糖苷 (GSL) 化合物的产生与 GSL 相关基因表达谱之间的时间关系。在整个生命周期中，萝卜硫素 (GRH) 和萝卜硫素 (GRE) 在萝卜中占主导地位，而大白菜中富含氨基葡萄糖苷（GNP）、芥子苷（GBN）和萝卜硫苷（GRA）。Baemoochae 包含所有研究的 GSL 的中间水平，表明从萝卜和大白菜遗传。BCAT4、CYP79F1、CYP83A1、UGT74B1、GRS1、FMOgs-ox1和AOP2基因的表达模式与萝卜、大白菜和白菜中相应的编码蛋白相关。有趣的是，在这些植物的营养和生殖阶段，参与侧链修饰的基因表达模式发生了急剧变化，特别是 GRS1、FMOgs-ox1 和 AOP2。例如，GRS1 在叶片发育过程中强烈表达，而 FMOgs-ox1 和 AOP2 在花组织中均高表达。此外，负责 GRH 产生的 GRS1 基因的表达主要在萝卜和白菜的叶组织中表达，而在大白菜根组织中仅少量检测到，这解释了为什么与其他芸苔属植物相比，萝卜具有丰富的 GRH。总之，我们的综合和比较数据证明，脂肪族 GSLs 的生物合成在十字花科家族成员中以组织和发育依赖的方式动态而精确地调节。