BACKGROUND Anti-neoplastic agents are widely used in the treatment of cancer and some non-neoplastic diseases. These drugs have been proved to be carcinogens, teratogens, and mutagens. Concern exists regarding the possible dangers of the staff handling anti-cancer drugs. The long-term exposure of nurses to anti-neoplastic drugs is still a controversial issue. The purpose of this study was to monitor cellular toxicity parameters and gene expression in nurses who work in chemotherapy wards and compare them to nurses who work in other wards. METHODS To analyze the apoptosis-related genes overexpression and cytotoxicity effects, peripheral blood lymphocytes obtained from oncology nurses and the control group. THE RESULTS Significant alterations in four analyzed apoptosis-related genes were observed in oncology nurses. In most individual samples being excavated, Bcl-2 overexpression is superior to that of Bax. Prominent P53 and Hif-1α up-regulation were observed in oncology nurses. Moreover, all cytotoxicity parameters (cell viability, ROS formation, MMP collapse, Lysosomal membrane damage, Lipid peroxidation, Caspase 3 activity and Apoptosis phenotype) in exposed oncology nurses were significantly (p < 0.001) higher than those of unexposed control nurses. Up-regulation of three analyzed apoptosis-related genes were observed in nurses occupationally exposed to anti-cancer drugs. CONCLUSION Our data show that oxidative stress and mitochondrial toxicity induced by anti-neoplastic drugs lead to overexpression of apoptosis-related genes in oncology nurses.

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暴露于抗肿瘤药物的护士中与凋亡相关的基因分析。

背景技术抗肿瘤剂被广泛用于治疗癌症和一些非肿瘤性疾病。这些药物已被证明是致癌物，致畸物和诱变剂。人们担心使用抗癌药的人员可能存在危险。护士长期接触抗肿瘤药物仍然是一个有争议的问题。这项研究的目的是监测在化学病房工作的护士的细胞毒性参数和基因表达，并将其与在其他病房工作的护士进行比较。方法分析肿瘤护理人员和对照组的外周血淋巴细胞，分析细胞凋亡相关基因的过表达和细胞毒性作用。结果在肿瘤科护士中观察到四个与凋亡相关的基因的显着改变。在挖掘的大多数单个样本中，Bcl-2的过表达优于Bax。在肿瘤科护士中观察到明显的P53和Hif-1α上调。此外，在暴露的肿瘤科护士中，所有细胞毒性参数（细胞活力，ROS形成，MMP塌陷，溶酶体膜损伤，脂质过氧化，Caspase 3活性和细胞凋亡表型）均显着（p <0.001）高于未暴露的对照护士。在职业性接触抗癌药物的护士中观察到三个分析的凋亡相关基因的上调。结论我们的数据表明抗肿瘤药物引起的氧化应激和线粒体毒性导致肿瘤护士凋亡相关基因的过表达。在肿瘤科护士中观察到明显的P53和Hif-1α上调。此外，在暴露的肿瘤科护士中，所有细胞毒性参数（细胞活力，ROS形成，MMP崩溃，溶酶体膜损伤，脂质过氧化，Caspase 3活性和细胞凋亡表型）均显着（p <0.001）高于未暴露的对照护士。在职业性接触抗癌药物的护士中观察到三个分析的凋亡相关基因的上调。结论我们的数据表明抗肿瘤药物引起的氧化应激和线粒体毒性导致肿瘤护士凋亡相关基因的过表达。在肿瘤科护士中观察到了明显的P53和Hif-1α上调。此外，在暴露的肿瘤科护士中，所有细胞毒性参数（细胞活力，ROS形成，MMP崩溃，溶酶体膜损伤，脂质过氧化，Caspase 3活性和细胞凋亡表型）均显着（p <0.001）高于未暴露的对照护士。在职业性接触抗癌药物的护士中观察到三个分析的凋亡相关基因的上调。结论我们的数据表明抗肿瘤药物引起的氧化应激和线粒体毒性导致肿瘤护士凋亡相关基因的过表达。裸露的肿瘤护士的Caspase 3活性和细胞凋亡表型显着高于未暴露的对照护士（p <0.001）。在职业性接触抗癌药物的护士中观察到三个分析的凋亡相关基因的上调。结论我们的数据表明抗肿瘤药物引起的氧化应激和线粒体毒性导致肿瘤护士凋亡相关基因的过表达。裸露的肿瘤护士的Caspase 3活性和细胞凋亡表型显着高于未暴露的对照护士（p <0.001）。在职业性接触抗癌药物的护士中观察到三个分析的凋亡相关基因的上调。结论我们的数据表明抗肿瘤药物引起的氧化应激和线粒体毒性导致肿瘤护士凋亡相关基因的过表达。