Mechanical interactions between muscle and bone have long been recognized as integral to bone integrity. However, few studies have directly measured these interactions within the context of musculoskeletal disease. In this study, the osteogenesis imperfecta murine model (oim/oim) was utilized because it has both reduced bone and muscle properties, allowing direct assessment of whether weakened muscle is able to engender strain on weakened bone. To do so, a strain gauge was attached to the tibia of healthy and oim/oim mice, muscles within the posterior quadrant of the lower hind limb were stimulated, and bone strain during muscle contraction was measured. Results indicated that the relationship between maximum muscle torque and maximum engendered strain is altered in oim/oim bone, with less torque required to engender strain compare to wild-type and heterozygous mice. Maximum muscle torque at 150 Hz stimulation frequency was able to engender ~1500 μɛ in oim/oim animals. However, even though the strain engendered in the oim/oim mice was high relative to historical bone formation thresholds, the maximum strain values were still significantly lower than that of the wild-type mice. These results are promising in that they suggest that muscle stimulation may be a viable means of inducing bone formation in oim/oim and potentially other disease models where muscle weakness/atrophy exist.

中文翻译：

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尽管在成骨不全的小鼠模型中肌肉无力，但肌肉收缩会诱导皮质骨应变的成骨水平

长期以来，肌肉和骨骼之间的机械相互作用被认为是骨骼完整性不可或缺的一部分。然而，很少有研究直接测量肌肉骨骼疾病背景下的这些相互作用。在这项研究中，使用了成骨不全鼠模型 (oim/oim)，因为它具有减少的骨骼和肌肉特性，可以直接评估减弱的肌肉是否能够对减弱的骨骼产生应变。为此，将应变计连接到健康和 oim/oim 小鼠的胫骨，刺激下后肢后象限内的肌肉，并测量肌肉收缩期间的骨应变。结果表明，在 oim/oim 骨骼中，最大肌肉扭矩和最大产生应变之间的关系发生了改变，与野生型和杂合子小鼠相比，产生应变所需的扭矩更小。在 150 Hz 刺激频率下的最大肌肉扭矩能够在 oim/oim 动物中产生 ~1500 μɛ。然而，即使 oim/oim 小鼠产生的应变相对于历史骨形成阈值较高，但最大应变值仍显着低于野生型小鼠。这些结果很有希望，因为它们表明肌肉刺激可能是在 oim/oim 和潜在的其他存在肌肉无力/萎缩的疾病模型中诱导骨形成的可行方法。最大应变值仍显着低于野生型小鼠。这些结果很有希望，因为它们表明肌肉刺激可能是在 oim/oim 和潜在的其他存在肌肉无力/萎缩的疾病模型中诱导骨形成的可行方法。最大应变值仍显着低于野生型小鼠。这些结果很有希望，因为它们表明肌肉刺激可能是在 oim/oim 和潜在的其他存在肌肉无力/萎缩的疾病模型中诱导骨形成的可行方法。