The Placenta, like every tissue, possesses its own characteristic protein profile, which may change within the course of pregnancy. These changes can be used for the elucidation of the mechanisms related to both physiology of pregnancy and pathological events. The aim of the study was to describe proteinergic profiles of maternal and fetal parts of bovine placenta during early-mid pregnancy by the use of 2D electrophoresis and MALDI TOF/TOF MS identification to evaluate dynamics of the possible changes necessary for placentation. Placental samples were collected from six pregnant cows (3-5 months) in the local abattoir. Placentomes were separated, and proteins were extracted and subjected to 2D electrophoresis and MALDI TOF/TOF identification. Out of 907 spots identified by the statistical analysis of gels, 54 were identified. Out of this number, 36 spots were significantly different between examined samples. Moreover, the obtained patterns differed between maternal and fetal parts of the placenta with regard to the intensity of staining, suggesting quantitative differences in protein content. These preliminary results are unique for this period of pregnancy. Such data are important for further experiments to obtain full protein profiles necessary to understand biochemical mechanisms underlying the attachment between fetal and maternal parts of the placenta during placentation. Moreover, the outcomes may help in elucidating pregnancy biomarkers in the future.

中文翻译：

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孕早期-中期牛胎盘蛋白的二维电泳图谱。

胎盘，像每个组织一样，都有自己的蛋白质特征，在怀孕过程中可能会发生变化。这些变化可用于阐明与妊娠生理和病理事件有关的机制。这项研究的目的是通过使用2D电泳和MALDI TOF / TOF MS鉴定来描述牛胎盘孕产妇和胎儿部分的蛋白质能谱，以评估胎盘可能发生的变化的动力学。从当地屠宰场的六头怀孕母牛（3-5个月）中收集胎盘样本。分离胎盘，提取蛋白质并进行2D电泳和MALDI TOF / TOF鉴定。在通过凝胶统计分析鉴定出的907个斑点中，有54个被鉴定出来。在这个数字之外，在检查的样品之间有36个斑点有显着差异。此外，在胎盘的母胎部分和胎盘部分之间，所获得的模式在染色强度方面也有所不同，表明蛋白质含量存在定量差异。这些初步结果在这个怀孕时期是独一无二的。这样的数据对于进一步的实验非常重要，以获得完整的蛋白质图谱，这对于理解胎盘在胎盘的胎儿和母体部分之间的附着所基于的生化机制是必要的。此外，结果可能有助于将来阐明妊娠生物标志物。表明蛋白质含量存在定量差异。这些初步结果在这个怀孕时期是独一无二的。这样的数据对于进一步的实验非常重要，以获得完整的蛋白质图谱，这对于理解胎盘在胎盘的胎儿和母体部分之间的附着所基于的生化机制是必不可少的。此外，结果可能有助于将来阐明妊娠生物标志物。表明蛋白质含量存在定量差异。这些初步结果在这个怀孕时期是独一无二的。这样的数据对于进一步的实验非常重要，以获得完整的蛋白质图谱，这对于理解胎盘在胎盘的胎儿和母体部分之间的附着所基于的生化机制是必不可少的。此外，结果可能有助于将来阐明妊娠生物标志物。