Abstract Air assisted dispersive liquid–liquid microextraction (AA-DLLME) using hydrophilic–hydrophobic deep eutectic solvents (DES) was developed for the simultaneous isolation of monosaccharides and amino acids with wide ranges of polarities from kelp using high performance liquid chromatography (HPLC). A response surface methodology (RSM) on a Box–Behnken design (BBD) model was employed to identify the optimal extraction parameters. Air assisted dispersive liquid-phase microextraction performed using the optimum deep eutectic solvent system, five push–pull cycles, a ratio of solid to liquid equal to 3 mg·mL−1, 10% (w/v) NaCl, and a centrifugation time of 6 min provided the best analytical performance. The optimal extracted concentrations of d-(+)-galactose, l-(-)-fucose, dl-tyrosine, and dl-valine in kelp were 16.7 ± 0.2, 8.6 ± 0.2, 2.6 ± 0.1, and 1.6 ± 0.1 mg·g−1, respectively. The method recoveries for d-(+)-galactose, l-(-)-fucose, dl-tyrosine, and dl-tyrosine were from 87 to 102%, 84 to 103%, 87 to 104%, and 85 to 103%. The relative standard deviations (RSDs) (n = 4) for the intra-day and inter-day determinations were <6.17%.

中文翻译：

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空气辅助分散液-液微萃取 (AA-DLLME) 使用亲水-疏水深共晶溶剂从海带中分离单糖和氨基酸

摘要 空气辅助分散液液微萃取 (AA-DLLME) 使用亲水-疏水深共熔溶剂 (DES) 被开发用于使用高效液相色谱 (HPLC) 从海带中同时分离具有广泛极性的单糖和氨基酸。Box-Behnken 设计 (BBD) 模型上的响应面方法 (RSM) 用于确定最佳提取参数。空气辅助分散液相微萃取使用最佳的低共熔溶剂系统、五个推拉循环、固液比等于 3 mg·mL−1、10% (w/v) NaCl 和离心时间6 分钟提供了最佳的分析性能。海带中d-(+)-半乳糖、l-(-)-岩藻糖、dl-酪氨酸和dl-缬氨酸的最佳提取浓度为16.7±0.2、8.6±0.2、2.6±0.1，和 1.6 ± 0.1 mg·g-1，分别。d-(+)-半乳糖、l-(-)-岩藻糖、dl-酪氨酸和 dl-酪氨酸的方法回收率分别为 87% 到 102%、84% 到 103%、87% 到 104% 和 85% 到 103% . 日内和日间测定的相对标准偏差 (RSD) (n = 4) <6.17%。