Abstract The goal of this study was to determine the content of 12 metals in 23 samples of teeth from two cities in Bosnia and Herzegovina (B&H): Sarajevo, a capital city with heavy traffic, industrial facilities, and long periods of smog during winter and Bihac, a picturesque small city, with no industrialization, settled among vivid national park and rivers. The teeth were separated into enamel and dentin. Dissolution of samples was performed in concentrated HNO3 with the addition of H2O2 followed by flame atomic absorption spectroscopy (FAAS) analysis. The results showed expected high contents of Ca, Na, Mg, and K, while elevated contents of Cu, Fe, and Zn were present in some samples. K and Na showed uniform distributions throughout enamel and dentin. Alkaline and earth-alkaline metals showed significant positive correlations. Zinc and manganese exhibited differences in the dentin content based on the place of residence. Zinc also displayed statistically significant differences between smokers’ and nonsmokers’ dentin samples. The differences were more pronounced between intra groups (within one sample) than for inter groups (within different groups, such as location, gender, and smoking).

中文翻译：

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人类牙釉质和牙本质：性别、地理位置和吸烟对金属浓度的影响

摘要 本研究的目的是确定波斯尼亚和黑塞哥维那 (B&H) 两个城市的 23 个牙齿样本中 12 种金属的含量：萨拉热窝，首都城市，交通繁忙，工业设施齐全，冬季和冬季长期烟雾缭绕。比哈奇是一座风景如画的小城市，没有工业化，坐落在生动的国家公园和河流之间。牙齿分为牙釉质和牙本质。在加入 H2O2 的浓 HNO3 中溶解样品，然后进行火焰原子吸收光谱 (FAAS) 分析。结果表明，Ca、Na、Mg 和 K 的预期含量较高，而某些样品中 Cu、Fe 和 Zn 的含量较高。K 和 Na 在整个牙釉质和牙本质中显示出均匀分布。碱金属和土碱金属呈显着正相关。锌和锰的牙本质含量因居住地而异。锌还在吸烟者和非吸烟者的牙本质样本之间显示出统计学上的显着差异。组内（一个样本内）的差异比组间（不同组内，例如位置、性别和吸烟）的差异更明显。