Abstract The aim of this study was to evaluate the formation of aqueous two-phase systems (ATPS) based on different protic ionic liquids (PIL), synthesized using amines, and organic or inorganic acid, and acetonitrile (ACN). The ATPSs are used to pre-purify genipin from genipap (Genipa americana L.). All PILs (purity above 98%) were able to form ATPSs with ACN, the size of the biphasic region being directly dependent on salting-out aptitude of PIL anions and relative hydrophilicity of the corresponding cations. The highest solid–liquid extraction of genipin from genipap was achieved by using aqueous ACN solution at 75% (16.79 ± 0.06 mg g−1 dry raw material) as extractant. After the extraction stage, the ATPS was used as an integrated platform to pre-purify the genipin present in the extract. Three different overall mixture point compositions were tested, and those formed by [MEA][H2Cit] (25 wt%) + ACN (45 wt%) + water (30 wt%) at 298.15 ± 1.00 K and 0.10 ± 0.01 MPa showed the highest selectivity (S = 4.202 ± 0.009) and recovery of genipin in top phase (RTG = 56.74 ± 0.04%), and the purification factor in top phase was 5.9 fold.

中文翻译：

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质子离子液体作为基于乙腈的水性两相系统的组成部分：合成、相图和京尼平预纯化

摘要 本研究的目的是评估基于不同质子离子液体 (PIL)、使用胺、有机或无机酸和乙腈 (ACN) 合成的水性两相系统 (ATPS) 的形成。ATPS 用于从 genipap (Genipa americana L.) 中预纯化京尼平。所有 PIL（纯度高于 98%）都能够与 ACN 形成 ATPS，双相区域的大小直接取决于 PIL 阴离子的盐析能力和相应阳离子的相对亲水性。使用 75% 的 ACN 水溶液（16.79 ± 0.06 mg g−1 干原料）作为萃取剂，从京尼帕中提取京尼平的最高固液萃取率。在提取阶段之后，ATPS 用作集成平台来预纯化提取物中存在的京尼平。