The production of microalgae-derived biodiesel without catalyst from partially wet microalgae (Chlorella vulgaris) by in situ cell disruption, extraction, and transesterification using methanol mixed with castor oil containing pressurized CO₂ was investigated. The effects of the operating variables on the yield of fatty acid methyl ester (FAME) were systematically examined. The addition of non-edible castor oil was observed to reduce operation pressure and time. Under the most appropriate conditions as a microalgae amount of 10 g containing 20 wt% water, a methanol-to-biomass ratio of 1.5 (g/g), a castor oil amount of 4.5 g, a temperature of 220 °C, a pressure of 9.7 MPa and a time of 30 min, the microalgae and castor oil FAME yields were 61.4% and 63.3%, respectively. The obtained results revealed that the proposed means could reduce energy and chemical waste because of higher water tolerance, lower methanol-to-microalgae ratio, lower operation pressure and shorter operation time.

通过原位细胞破碎，萃取和酯交换反应，使用甲醇与含加压CO _2的蓖麻油混合，从部分湿润的微藻类（小球藻）生产不含催化剂的微藻类生物柴油。被调查了。系统地检查了操作变量对脂肪酸甲酯（FAME）产量的影响。观察到添加了非食用蓖麻油以减少操作压力和时间。在最合适的条件下，微藻量为10 g，其中包含20 wt％的水，甲醇生物量比为1.5（g / g），蓖麻油量为4.5 g，温度为220°C，压力为在9.7 MPa和30分钟的时间下，微藻和蓖麻油的FAME产率分别为61.4％和63.3％。所得结果表明，该方法具有较高的耐水性，较低的甲醇与微藻比例，较低的操作压力和较短的操作时间，因此可以减少能源和化学废物。