Virtually all organisms respond to heat shock by transcription of genes encoding for heat shock proteins (HSPs), but the mechanisms behind post-transcriptional regulation are not known in detail. When we exposed zebrafish to 5 and 7 °C above normal rearing temperature for 30 min, hsp70 mRNA expression was 28 and 150 -fold higher than in control, respectively. Protein expression, on the other hand, showed no significant change at the +5 °C and a 2-fold increase at the +7 °C exposure. This suggests that the transcription of hsp70 gene does not immediately correspond to translation to related proteins under certain stress temperatures, but, when the temperature is higher, and potentially detrimental, transcription and translation are intimately coupled. Those results confirm that temperature is an important abiotic factor involved in heat shock post-transcriptional regulation mechanisms in fish. However, further studies are needed to determine the relationship between this environmental factor and post-transcriptional regulation mechanisms. Earlier, the coupling/uncoupling of hsp transcription and translation has only been studied using cold-water fish, or zebrafish embryos. With current findings, we suggest this mechanism might be present even in adult warm water fish like the zebrafish.

中文翻译：

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Hsp70s转录-翻译关系取决于斑马鱼的热激温度。

几乎所有生物体都通过编码热激蛋白（HSP）的基因转录来对热激作出反应，但是转录后调控的机制尚不清楚。当我们使斑马鱼在高于正常饲养温度的5和7°C下暴露30分钟时，hsp70 mRNA的表达分别比对照高28和150倍。另一方面，蛋白质表达在+5°C时无明显变化，而在+7°C时无2倍的增加。这表明在某些胁迫温度下，hsp70基因的转录并不立即对应于相关蛋白的翻译，而是当温度更高时，可能有害的转录和翻译紧密结合。这些结果证实温度是鱼类热休克转录后调控机制中的重要非生物因子。但是，需要进一步的研究来确定这种环境因素与转录后调控机制之间的关系。早先，仅使用冷水鱼或斑马鱼胚胎研究了hsp转录和翻译的偶联/解偶联。根据目前的发现，我们建议即使在成年温水鱼（如斑马鱼）中也可能存在这种机制。或斑马鱼的胚胎。根据目前的发现，我们建议即使在成年温水鱼（如斑马鱼）中也可能存在这种机制。或斑马鱼的胚胎。根据目前的发现，我们建议即使在成年温水鱼（如斑马鱼）中也可能存在这种机制。