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Mechano-Optical Analysis of Single Cells with Transparent Microcapillary Resonators.
ACS Sensors ( IF 8.2 ) Pub Date : 2019-12-12 , DOI: 10.1021/acssensors.9b02038
Alberto Martín-Pérez 1 , Daniel Ramos 1 , Eduardo Gil-Santos 1 , Sergio García-López 1 , Marina L Yubero 1 , Priscila M Kosaka 1 , Álvaro San Paulo 1 , Javier Tamayo 1 , Montserrat Calleja 1
Affiliation  

The study of biophysical properties of single cells is becoming increasingly relevant in cell biology and pathology. The measurement and tracking of magnitudes such as cell stiffness, morphology, and mass or refractive index have brought otherwise inaccessible knowledge about cell physiology, as well as innovative methods for high-throughput label-free cell classification. In this work, we present hollow resonator devices based on suspended glass microcapillaries for the simultaneous measurement of single-cell buoyant mass and reflectivity with a throughput of 300 cells/minute. In the experimental methodology presented here, both magnitudes are extracted from the devices' response to a single probe, a focused laser beam that enables simultaneous readout of changes in resonance frequency and reflected optical power of the devices as cells flow within them. Through its application to MCF-7 human breast adenocarcinoma cells and MCF-10A nontumorigenic cells, we demonstrate that this mechano-optical technique can successfully discriminate pathological from healthy cells of the same tissue type.

中文翻译:

带有透明微毛细管谐振器的单电池的机械光学分析。

单细胞生物物理特性的研究在细胞生物学和病理学中变得越来越重要。诸如细胞刚度,形态,质量或折射率之类的大小的测量和跟踪带来了其他有关细胞生理学的难以理解的知识,以及用于高通量无标签细胞分类的创新方法。在这项工作中,我们介绍了基于悬浮玻璃微毛细管的中空谐振器设备,用于同时测量单细胞浮力和反射率,吞吐率为300个细胞/分钟。在此处介绍的实验方法中,两个幅度都是从设备对单个探头的响应中提取的,聚焦的激光束,使得能够同时读出共振频率的变化以及随着细胞在其中流动而产生的反射光功率。通过将其应用于MCF-7人乳腺腺癌细胞和MCF-10A非致瘤细胞,我们证明了这种机械光学技术可以成功地将病理学与相同组织类型的健康细胞区分开。
更新日期:2019-12-13
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