Argonaute-2 (Ago2) is a key component of the RNA-induced silencing complex that mediates downregulation of mRNA by miRNAs. Its presence in extracellular vesicles (EV) has been postulated to be important for the activity of EV-carried miRNA in modulating gene expression in recipient cells. However, whether it is in fact contained within EVs or is instead an extravesicular contaminant is controversial. In this opinion piece, we argue that the ability to detect Ago2 in EVs is a result of multiple factors, including cell source, cell signaling control of Ago2 trafficking to EVs, experimental conditions, and detection methods.

中文翻译：

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细胞外小囊中的Argonautes：是人工制品还是特选货物？

Argonaute-2（Ago2）是RNA诱导的沉默复合体的关键成分，该复合体介导miRNA下调mRNA的表达。据推测，它在细胞外囊泡（EV）中的存在对于EV携带的miRNA在调节受体细胞中的基因表达中的活性很重要。但是，究竟是将其包含在电动汽车中，还是将其作为囊外污染物存在争议。在这篇观点中，我们认为在电动汽车中检测Ago2的能力是多种因素的结果，包括细胞来源，对Ago2贩运至电动汽车的细胞信号控制，实验条件和检测方法。