Here we established a palm‐sized electrochemical sensor for the determination of cell‐released superoxide anions (O₂^.−) based on a self‐developed portable potentiostat (xenSTAT) which can perform fast analysis with visual real‐time data and customizable parameters. The xenSTAT was equipped with Bluetooth and USB interfaces, thus being able to be connected with both smartphones and computers for electrochemical analysis. A novel Nanozyme based on Mn_x(PO₄)_y monolayer modified porous carbon cubic was synthesized and used as functional material on the surface of working electrode. By using cyclic voltammetry (CV) and chronoamperometry, xenSTAT was demonstrated to exhibit satisfying sensitivity and excellent stability for real‐time monitoring of O₂^.− released from cancer cells. With decreased cost, customizable development, small size and convenient usage, the xenSTAT described in this work could promote the commercialization and widespread of in‐house built electrochemical sensors for the monitoring of O₂^.− in the future.

中文翻译：

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基于Mnx（PO4）y单层修饰多孔碳的智能手机耦合的细胞超氧阴离子电化学分析

在这里，我们基于自行开发的便携式恒电位仪（xenSTAT）建立了一种掌上型电化学传感器，用于测定细胞释放的超氧阴离子（O ₂ ^.-），该传感器可以通过视觉实时数据和可自定义的参数进行快速分析。xenSTAT配备了蓝牙和USB接口，因此可以与智能手机和计算机连接进行电化学分析。一种基于Mn _x（PO ₄）_y的新型纳米酶合成了单层改性多孔碳立方，并用作工作电极表面的功能材料。通过使用循环伏安法（CV）和计时电流法，xenSTAT被证明对实时监测从癌细胞释放的O ₂ ^.-表现出令人满意的灵敏度和出色的稳定性。随着成本的降低，可定制的开发，小巧的尺寸和方便的使用，这项工作中描述的xenSTAT可以促进内部监测O ₂ ^.-的内置电化学传感器的商业化和广泛应用。