BACKGROUND In recent decades, the frequency of flooding is increasing with the change of global climate. Flooding has become one of the major abiotic stresses that seriously affect growth and development of plants. Triarrhena sacchariflora Nakai has been considered a promising energy crop for utilization in ethanol production. Flooding stress is among the most severe abiotic stressors in the production of Nakai. However, the physiological and molecular biological mechanisms of Nakai response to flooding is still unclear. In the present study, in order to understand the molecular mechanisms of Nakai in response to flooding stress, the transcriptome, physiological and biochemical were investigated. RESULTS The results demonstrated that significant physiological changes were observed in photosynthetic system, antioxidative enzyme activity, chlorophyll, carotenoid, proline, lipid peroxidation and soluble sugar content under normal and flooding treatments. Such as, the chlorophyll, carotenoid contents and photosynthetic system were significantly decreased. Whereas, the antioxidative enzyme activity, proline, lipid peroxidation and soluble sugar has increased first and then decreased under treatments compared with the normal plants. Additionally, a total of 8832, 6608 and 3649 unigenes were validated to be differentially expressed under different treatments, respectively. Besides, gene ontology enrichment and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis of the different expression levels of genes also presented processes, which involved in photosynthesis, sucrose catabolism, glycolysis, stress response and defense, phytohormone biosynthesis and signal transduction. CONCLUSIONS The results provide a comprehensive view of the complex molecular events involved in the response to flooding stress of Nakai leaves, which also will promote the research in the development of flood-resistant crops and provide new tools for Nakai breeders.

中文翻译：

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满江红响应水淹胁迫的转录组学，生理生化分析。

背景技术在最近的几十年中，随着全球气候的变化，洪水泛滥的频率正在增加。洪水已成为严重影响植物生长发育的主要非生物胁迫之一。酒（Triarrhena sacchariflora Nakai）被认为是一种有前途的能源作物，可用于乙醇生产。洪水压力是Nakai生产中最严重的非生物压力源之一。但是，中井对洪水的生理和分子生物学机制仍不清楚。在本研究中，为了了解中井响应水淹胁迫的分子机制，研究了转录组，生理生化。结果结果表明，光合系统，抗氧化酶活性，在正常和淹水处理下，叶绿素，类胡萝卜素，脯氨酸，脂质过氧化作用和可溶性糖含量。例如，叶绿素，类胡萝卜素含量和光合系统明显降低。而与正常植物相比，抗氧化酶活性，脯氨酸，脂质过氧化和可溶性糖含量先升高后降低。此外，分别验证了总共8832、6608和3649个单基因在不同处理下的差异表达。此外，基因本体的富集以及《京都议定书》和《基因组通路全书》对不同基因表达水平的分析也提出了过程，这些过程涉及光合作用，蔗糖分解代谢，糖酵解，应激反应和防御，植物激素的生物合成和信号转导。结论该结果提供了一个综合的分子事件的综合视图，这些分子事件涉及对Nakai叶片的水淹胁迫的响应，这也将促进抗水作物的研究，并为Nakai育种者提供新的工具。