We describe ReorientExpress, a method to perform reference-free orientation of transcriptomic long sequencing reads. ReorientExpress uses deep learning to correctly predict the orientation of the majority of reads, and in particular when trained on a closely related species or in combination with read clustering. ReorientExpress enables long-read transcriptomics in non-model organisms and samples without a genome reference without using additional technologies and is available at https://github.com/comprna/reorientexpress.

中文翻译：

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ReorientExpress：通过深度学习实现纳米孔 cDNA 读取的无参考定向


我们描述了 ReorientExpress，一种对转录组长测序读数进行无参考定向的方法。 ReorientExpress 使用深度学习来正确预测大多数读取的方向，特别是在密切相关的物种上进行训练或与读取聚类相结合时。 ReorientExpress 无需使用其他技术即可在非模型生物体和样品中进行长读转录组学，无需基因组参考，可从 https://github.com/comprna/reorientexpress 获取。