Identifying the variables that drive tamoxifen-independent CreERT2 recombination: Implications for microglial fate mapping and gene deletions.,European Journal of Immunology

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Identifying the variables that drive tamoxifen-independent CreERT2 recombination: Implications for microglial fate mapping and gene deletions.
European Journal of Immunology ( IF 4.5 ) Pub Date : 2019-12-11 , DOI: 10.1002/eji.201948162
Hannah Van Hove _{1,

2} , Ana Rita Pombo Antunes _{1,

2} , Karen De Vlaminck _{1,

2} , Isabelle Scheyltjens _{1,

2} , Jo A Van Ginderachter _{1,

2} , Kiavash Movahedi _{1,

2}

Affiliation

Ligand-dependent Cre recombinases such as the CreERT2 system allow for tamoxifen-inducible Cre recombination. Important examples are the Cx3cr1-CreERT2 and Sall1-CreERT2 lines that are widely used for fate mapping and gene deletion studies of brain macrophages. Our results now show that both CreERT2 lines can exhibit a high rate of tamoxifen-independent "leaky" excision with some reporter strains, while this is not observed with others. We suggest that this disparity is determined by the length of the floxed transcriptional STOP cassette that is incorporated in the various reporter lines. In addition, the rate of spontaneous recombination was also determined by the CreERT2 expression levels and the longevity of the CreERT2-expressing cells. The implications of these results are discussed in the context of fate mapping and inducible gene deletion studies in macrophages and microglia.

中文翻译：

识别驱动他莫昔芬独立的CreERT2重组的变量：对小胶质细胞命运定位和基因删除的影响。

依赖配体的Cre重组酶（例如CreERT2系统）允许他莫昔芬诱导的Cre重组。重要的例子是Cx3cr1-CreERT2和Sall1-CreERT2系，这些系被广泛用于脑巨噬细胞的命运定位和基因删除研究。我们的结果现在表明，在某些报告基因菌株中，两个CreERT2品系均具有较高的他莫昔芬非依赖性“泄漏”切除率，而在其他报告基因菌株中未观察到。我们建议，这种差异是由掺入各种报道基因系中的转录转录STOP盒的长度决定的。另外，自发重组的速率也由CreERT2表达水平和表达CreERT2的细胞的寿命决定。

更新日期：2019-12-11

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