Primary seed dormancy is a mechanism that orchestrates the timing of seed germination in order to prevent out-of-season germination. Secondary dormancy can be induced in imbibed seeds when they encounter prolonged unfavourable conditions. Secondary dormancy is not induced during dry storage, and therefore the mechanisms underlying this process have remained largely unexplored. Here, a 2-year seed burial experiment in which dormancy cycling was studied at the physiological and transcriptional level is presented. For these analyses six different Arabidopsis thaliana genotypes were used: Landsberg erecta (Ler) and the dormancy associated DELAY OF GERMINATION (DOG) near-isogenic lines 1, 2, 3, 6 and 22 (NILDOG1, 2, 3, 6 and 22). The germination potential of seeds exhumed from the field showed that these seeds go through dormancy cycling and that the dynamics of this cycling is genotype dependent. RNA-seq analysis revealed large transcriptional changes during dormancy cycling, especially at the time points preceding shifts in dormancy status. Dormancy cycling is driven by soil temperature and the endosperm is important in the perception of the environment. Genes that are upregulated in the low- to non-dormant stages are enriched for genes involved in translation, indicating that the non-dormant seeds are prepared for rapid seed germination.

中文翻译：

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休眠循环：翻译相关的转录本是田间休眠种子和非休眠种子之间的主要区别。

初级种子休眠是一种协调种子发芽时间以防止反季发芽的机制。当吸收的种子遇到长时间的不利条件时，可以诱导其继发休眠。在干燥储存过程中不会引起次生休眠，因此，该过程的潜在机制在很大程度上仍未得到探索。在这里，提出了一个为期2年的种子埋葬实验，该实验在生理和转录水平研究了休眠循环。对于这些分析，使用了六种不同的拟南芥基因型：Landsberg erecta（Ler）和休眠相关的DELAY OF GERMINATION（DOG）近等基因系1、2、3、6和22（NILDOG1、2、3、6和22） 。从田间挖掘出的种子的发芽潜力表明，这些种子经历了休眠循环，并且该循环的动力学取决于基因型。RNA-seq分析显示，在休眠周期中，尤其是在休眠状态发生变化之前的时间点，转录变化很大。休眠循环是由土壤温度驱动的，而胚乳在感知环境方面很重要。在低休眠期至非休眠期中上调的基因富含用于翻译的基因，这表明非休眠种子已为种子快速发芽做好了准备。休眠循环是由土壤温度驱动的，而胚乳在感知环境方面很重要。在低休眠期至非休眠期中上调的基因富含用于翻译的基因，这表明非休眠种子已为种子快速发芽做好了准备。休眠循环是由土壤温度驱动的，而胚乳在感知环境方面很重要。在低休眠期至非休眠期中上调的基因富含用于翻译的基因，这表明非休眠种子已为种子快速发芽做好了准备。