Parastrephia lucida (Compositae), Tessaria absinthioides (Compositae), and Ephedra multiflora (Ephedraceae), three plant species from the Argentinean Puna (3600 m.a.s.l.) were selected for their anti-inflammatory and antioxidant properties to prepare mixtures to evaluate their use as nutraceuticals. Seven binary and ternary herbal mixtures made of ethanol 20% extracts of the selected plant species were prepared (Mixtures A to G), and they were analyzed for their effect on proinflammatory enzymes and their antioxidant activity in two cellular systems and in cell free systems. Toxicity tests were also carried out, and they were analyzed by high-performance liquid chromatography with a diode-array detector (HPLC-DAD) to quantify chemical markers. Mix A (equal parts of the three selected plant species) showed an important inhibitory capacity of different proinflammatory enzymes. Its potency on COX-2 was also higher than that of ibuprofen. Mix A and Mix G (P. lucida and T. absinthioides 1:1) showed a high antioxidant capacity in cellular and in cell-free systems. Toxicity assays further demonstrated their safety. This work shows the potential use of herbal mixtures made of medicinal plant species from the Argentinean Puna as nutraceutical or dietary supplements with antioxidant and anti-inflammatory activities. PRACTICAL APPLICATION: P. lucida, T. absinthioides, and E. multiflora are three plant species that are commonly used by Argentinean Puna inhabitants with medicinal purposes. Their proven safety, their antioxidant activity as well as their capacity to inhibit different proinflammatory enzymes make them attractive candidates to be used in combination as part of a dietary supplement aimed to prevent or palliate gastrointestinal and systemic inflammatory diseases. The use of native plant species as an alternative to more common and commercial plant species would have a positive impact on local communities' economies.

中文翻译：

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具有体外抗氧化和抗炎活性的阿根廷普纳植物作为潜在的营养品

选择了来自阿根廷普纳 (3600 masl) 的三种植物物种 Parastrephia lucida (Compositae)、Tessaria absinthioides (Compositae) 和 Ephedra multiflora (Ephedraceae)，以制备混合物以评估它们作为营养品的用途。制备了由所选植物物种的 20% 乙醇提取物制成的七种二元和三元草药混合物（混合物 A 到 G），并分析了它们对促炎酶的影响及其在两个细胞系统和无细胞系统中的抗氧化活性。还进行了毒性测试，并通过配备二极管阵列检测器 (HPLC-DAD) 的高效液相色谱法对化学标记物进行定量分析。混合物 A（三种选定植物物种的等份）显示出对不同促炎酶的重要抑制能力。它对 COX-2 的效力也高于布洛芬。Mix A 和 Mix G（P. lucida 和 T. absinthioides 1:1）在细胞和无细胞系统中显示出高抗氧化能力。毒性试验进一步证明了它们的安全性。这项工作显示了由来自阿根廷普纳的药用植物物种制成的草药混合物作为具有抗氧化和抗炎活性的营养保健品或膳食补充剂的潜在用途。实际应用：P. lucida、T. absinthioides 和 E. multiflora 是阿根廷普纳居民常用的三种药用植物。它们经过验证的安全性，它们的抗氧化活性以及抑制不同促炎酶的能力使它们成为有吸引力的候选物，可作为膳食补充剂的一部分联合使用，以预防或减轻胃肠道和全身炎症性疾病。使用本地植物物种作为更常见和商业植物物种的替代品将对当地社区的经济产生积极影响。