Au-Se bond strategy has been applied to construct fluorescence nanoprobe that is being used to simultaneously dual-image the tumor markers matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-7 (MMP-7) in vitro. The two Se-terminating ligand peptide chains respectively labeled with fluorescein isothiocyanate (FITC) and 5-Carboxytetramethylrhodamine (5-TAMRA) dyes are attached to the surface of Au nanoparticles (NPs). The nanoprobe can be specifically cut off by MMP-2 and MMP-7 respectively to carry out significant enhanced fluorescence recovery for simultaneously imaging both markers. The nanoprobe not only displays high selectivity and sensitivity towards MMP-2 and MMP-7, but also has strong anti-interference stability against biothiols and enhanced fidelity for avoiding ‘false positive’ results. This approach offers new perspectives in designing tumor-related early diagnosis approaches and also provides strategies for clinical applications.

中文翻译：

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通过Au-Se构建的荧光纳米探针对MMP-2和MMP-7进行同时生物成像

Au-Se键策略已被用于构建荧光纳米探针，该探针被用于同时在体外对肿瘤标记物基质金属蛋白酶2（MMP-2）和基质金属蛋白酶7（MMP-7）进行双重成像。分别用异硫氰酸荧光素（FITC）和5-羧四甲基罗丹明（5-TAMRA）染料标记的两条Se端基配体肽链附着在Au纳米颗粒（NPs）的表面上。可以分别通过MMP-2和MMP-7特异性截断纳米探针，以显着提高荧光回收率，以同时对两种标记物进行成像。纳米探针不仅显示出对MMP-2和MMP-7的高选择性和敏感性，而且对生物硫醇具有很强的抗干扰稳定性，并增强了保真度，从而避免了“假阳性”结果。这种方法为设计与肿瘤相关的早期诊断方法提供了新的视角，也为临床应用提供了策略。